Dvorák B, Kolínská J, McWilliam D L, Williams C S, Higdon T, Zákostelecká M, Koldovský O
Department of Pediatrics and Steele Memorial Children's Research Center, University of Arizona College of Medicine, Tucson 85724-5073, USA.
FEBS Lett. 1998 Sep 11;435(1):119-24. doi: 10.1016/s0014-5793(98)01050-3.
Epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) are associated with regulation of various gastrointestinal functions. In order to better understand their role in developing small intestine EGF, TGF-alpha and EGF-R steady-state mRNA levels and transcript stability were determined. Reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed that intestinal TGF-alpha mRNA levels were 10-fold higher in comparison with EGF mRNA. The primary intestinal culture technique was used to evaluate mRNA stability. The stability of TGF-alpha mRNA was remarkably lower than the stability of EGF mRNA. High levels of TGF-alpha mRNA accompanied by high degradation rate of this mRNA suggested a rapid turnover of intestinal TGF-alpha mRNA.
表皮生长因子（EGF）和转化生长因子-α（TGF-α）与多种胃肠功能的调节有关。为了更好地了解它们在小肠发育中的作用，我们测定了EGF、TGF-α和EGF-R的稳态mRNA水平以及转录本稳定性。逆转录（RT）竞争性聚合酶链反应（PCR）显示，肠道TGF-α mRNA水平比EGF mRNA高10倍。采用原代肠道培养技术评估mRNA稳定性。TGF-α mRNA的稳定性显著低于EGF mRNA。TGF-α mRNA的高水平及其高降解率表明肠道TGF-α mRNA周转迅速。
