Morris J F, Peacock M
Clin Chim Acta. 1976 Nov 1;72(3):383-91. doi: 10.1016/0009-8981(76)90202-3.
A radio-competitive protein-binding assay for 25-hydroxy vitamin D3 (25-OHD3) is described using diluted plasma from patients with vitamin D deficient osteomalacia as the binding protein. The 25-OHD3 is extracted from plasma with ethanol and assayed directly without further purification. An incubation of two hours is used to give suitable binding. The lower detection limit of the assay is approximately 23 pg, the intra-assay coefficient of variation is 5.4% and the inter-assay variation 15%. Tth a mean of 26.9 mug/l. Levels of plasma 25-OHD3 are undetectable in patients with vitamin D deficient osteomalacia and greater than 110 mug/l in patients taking large doses of vitamin D2.
描述了一种用于检测25-羟维生素D3（25-OHD3）的放射竞争性蛋白结合测定法，该方法使用维生素D缺乏性骨软化症患者的稀释血浆作为结合蛋白。25-OHD3用乙醇从血浆中提取，无需进一步纯化即可直接测定。采用两小时孵育以实现合适的结合。该测定法的检测下限约为23皮克，批内变异系数为5.4%，批间变异为15%。平均值为26.9微克/升。维生素D缺乏性骨软化症患者血浆25-OHD3水平检测不到，而大剂量服用维生素D2的患者血浆25-OHD3水平大于110微克/升。
