Zhan X, Agrawal D K, Thorpe P E
Department of Radiology, Creighton University Medical Center, Omaha, NE 68131, USA.
J Vasc Interv Radiol. 1998 Sep-Oct;9(5):808-16. doi: 10.1016/s1051-0443(98)70396-3.
To investigate the influence of contrast media (CM) on endothelial cells (ECs) with respect to cytotoxicity and to neutrophil adhesion.
Human umbilical vein ECs were incubated with chromium-51-labeled human neutrophils in the presence of CM (diatrizoate, ioxaglate, iopamidol, and iodixanol) in three concentrations: 2, 20, and 50 mg I/mL. CM was compared with glucose solutions prepared from plain, buffered glucose solutions, iso-osmolar to the corresponding CM solution. Neutrophil adhesion to the EC monolayer, EC morphology, and cytotoxicity were evaluated.
The effect of CM on neutrophil adhesion was dependent on dose, with increased adhesion at low CM concentrations (2 and 20 mg I/mL) and decreased adhesion at high CM concentration (50 mg I/mL). The response was observed only if ECs and neutrophils were exposed to CM simultaneously in a shared environment. Glucose solutions with the same osmolarity did not show similar effect. Both diatrizoate and ioxaglate had a greater cytotoxic effect on ECs and neutrophils than did iodixanol and iopamidol.
The altered neutrophil adhesion to ECs may be due to CM-induced cytotoxicity or CM-induced EC activation because the glucose solutions did not cause a similar change at equal osmolality. The lack of cell death, combined with altered neutrophil adhesion implies modulation of cell adhesion molecules by CM. The results could be pertinent to the pathogenesis of peripheral vascular lesions and the endothelial response in immunosuppressed or septic patients receiving CM during imaging studies.
研究造影剂（CM）对内皮细胞（ECs）细胞毒性及中性粒细胞黏附的影响。
将人脐静脉内皮细胞与铬-51标记的人中性粒细胞共同培养，加入三种浓度（2、20和50 mg I/mL）的CM（泛影葡胺、碘克沙醇、碘帕醇和碘克酸）。将CM与由普通葡萄糖溶液、缓冲葡萄糖溶液制备的葡萄糖溶液进行比较，这些葡萄糖溶液与相应的CM溶液等渗。评估中性粒细胞对内皮细胞单层的黏附、内皮细胞形态及细胞毒性。
CM对中性粒细胞黏附的影响取决于剂量，低浓度CM（2和20 mg I/mL）时黏附增加，高浓度CM（50 mg I/mL）时黏附减少。仅当内皮细胞和中性粒细胞在共同环境中同时暴露于CM时才观察到该反应。等渗的葡萄糖溶液未显示出类似效果。与碘克酸和碘帕醇相比，泛影葡胺和碘克沙醇对内皮细胞和中性粒细胞的细胞毒性更大。
中性粒细胞对内皮细胞黏附的改变可能是由于CM诱导的细胞毒性或CM诱导的内皮细胞活化，因为等渗的葡萄糖溶液未引起类似变化。细胞死亡的缺乏，结合中性粒细胞黏附的改变，意味着CM对细胞黏附分子的调节。这些结果可能与外周血管病变的发病机制以及免疫抑制或脓毒症患者在影像学检查期间接受CM时的内皮反应有关。
