Dao-Thi M H, Rizkallah P, Wyns L, Poortmans F, Loris R
Laboratorium voor Ultrastruktuur, Vlaams Interinuversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium.
Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):844-7. doi: 10.1107/s0907444998001218.
The chitobiose specific Ulex europaeus lectin II crystallizes in space group P3221 with unit-cell dimensions a = b = 105.54, c = 176.26 A. The asymmetric unit contains a complete lectin tetramer. The crystals were shown to diffract to 4.5 A on a rotating-anode source and to 2.7 A at the Daresbury synchrotron source. Molecular replacement and subsequent rigid-body refinement using data to 4.5 A yielded a solution corresponding to a tetramer very similar to that of phytohemagglutinin-L and soybean agglutinin. The monomers in the Ulex lectin tetramer are rotated approximately 5 degrees compared with the phytohemagglutinin-L and soybean agglutinin structures.
壳二糖特异性荆豆凝集素II在空间群P3221中结晶，晶胞参数a = b = 105.54，c = 176.26 Å。不对称单元包含一个完整的凝集素四聚体。这些晶体在旋转阳极光源上衍射至4.5 Å，在达累斯伯里同步辐射光源上衍射至2.7 Å。利用4.5 Å的数据进行分子置换和随后的刚体精修，得到了一个与四聚体相对应的解决方案，该四聚体与植物血凝素-L和大豆凝集素的四聚体非常相似。与植物血凝素-L和大豆凝集素结构相比，荆豆凝集素四聚体中的单体旋转了约5度。
