Furuya H, Aikawa H, Hagino T, Yoshida T, Sakabe K
Department of Environmental Health, Tokai University School of Medicine, Kanagawa, Japan.
Nihon Eiseigaku Zasshi. 1998 Jul;53(2):420-5. doi: 10.1265/jjh.53.420.
The cellular effects of an extremely-low-frequency (ELF) magnetic field on mouse spermatogenesis were assessed by DNA flow cytometry and serum testosterone. Seven week old male ICR mice were exposed to a 50 Hz magnetic field the strength of which was 1.0 m Tesla. Seven mice per treatment group were exposed for 13, 26, 39 or 52 days. For each experimental point, an equal number of mice per sham-treated group were used as a control and were exposed only to the background field below 1 mu Tesla in the same room as the treatment group. In the control mice, the testis cellular DNA content distribution by flow cytometory was characterized by four quantifiable populations; round spermatids (1C), spermatogonia and other diploid cells (2C), spermatogonial cells synthesizing DNA (S-phase) and primary spermatocytes (4C). In animals exposed for 26 days the number of cells in the 4C and the 4C:2C ratio was significantly lower, and the 1C:4C ratio (meiotic transformation) was significantly higher than the corresponding control groups. In animals exposed for 52 days the cell population in 1C and the 1C:2C ratio (total germ-cell transformation) was significantly higher, and the cell population in 2C was significantly lower than the corresponding control groups. The concentration of serum testosterone in animals exposed for 13 days was significantly higher than in the corresponding control group. These changes suggest that long-term exposure to an ELF magnetic field had a possible effect on the proliferation and differentiation of spermatogonia.
通过DNA流式细胞术和血清睾酮评估极低频（ELF）磁场对小鼠精子发生的细胞效应。将7周龄雄性ICR小鼠暴露于强度为1.0毫特斯拉的50赫兹磁场中。每个处理组7只小鼠，分别暴露13、26、39或52天。对于每个实验点，每个假处理组使用相同数量的小鼠作为对照，它们仅在与处理组相同的房间内暴露于低于1微特斯拉的背景磁场中。在对照小鼠中，通过流式细胞术检测到的睾丸细胞DNA含量分布具有四个可量化的群体：圆形精子细胞（1C）、精原细胞和其他二倍体细胞（2C）、正在合成DNA的精原细胞（S期）和初级精母细胞（4C）。暴露26天的动物中，4C细胞数量和4C:2C比值显著降低，1C:4C比值（减数分裂转化）显著高于相应对照组。暴露52天的动物中，1C细胞群体和1C:2C比值（总生殖细胞转化）显著升高，2C细胞群体显著低于相应对照组。暴露13天的动物血清睾酮浓度显著高于相应对照组。这些变化表明，长期暴露于ELF磁场可能对精原细胞的增殖和分化产生影响。
