Trentz O A, Platz A, Helmy N, Trentz O
Forschungsabteilung Chirurgie.
Swiss Surg. 1998(4):203-9.
The biocompatibility of bone implants and substitutes is usually tested on cell-lines only, despite a different and more relevant behaviour on primary osteoblasts as final targets can be expected. "Osteoblast-like" cell line (MC3T3-E1) and fresh human osteoblasts (HOB), cultured from cancellous bone grafts from the iliac crest were used for the study. Three different clinically used biomaterials were compared regarding biocompatibility: titanium, steel and hydroxyapatite (Bio-Oss). "Osteoblast-like" cell line and fresh human osteoblasts (5x104) were seeded on the three bone implants. Cell proliferation and osteocalcin synthesis were determined 1, 3, 7, and 10 days after the cells were plated on the biomaterials. All experiments were performed in five times (pro culture double measurements). HOB proliferation on hydroxyapatite was decreasing after 3 days, whereas cells from "Osteoblast-like" cell line showed comparable proliferation to the control group. The most interesting observation was the significant decrease of the osteocalcin levels (in conditioned medium) of "osteoblast like" cells and HOB on HA (Bio-Oss). We conclude that HA disturbs the proliferation and osteocalcin synthesis of HOB.
尽管预计骨植入物和替代物在作为最终靶标的原代成骨细胞上会有不同且更相关的行为，但通常仅在细胞系上测试其生物相容性。本研究使用了从髂嵴松质骨移植培养的“成骨细胞样”细胞系（MC3T3-E1）和新鲜人成骨细胞（HOB）。比较了三种临床上常用的生物材料在生物相容性方面的差异：钛、钢和羟基磷灰石（Bio-Oss）。将“成骨细胞样”细胞系和新鲜人成骨细胞（5×10⁴）接种到三种骨植入物上。在细胞接种到生物材料上1、3、7和10天后，测定细胞增殖和成骨钙素合成。所有实验均进行了五次（原代培养重复测量两次）。3天后，HOB在羟基磷灰石上的增殖下降，而“成骨细胞样”细胞系的细胞增殖与对照组相当。最有趣的观察结果是，“成骨细胞样”细胞和HOB在HA（Bio-Oss）上（条件培养基中）的骨钙素水平显著下降。我们得出结论，HA会干扰HOB的增殖和成骨钙素合成。
