Karagouni E E, Chryssikopoulos A, Mantzavinos T, Kanakas N, Dotsika E N
Hellenic Pasteur Institute, Athens, Greece.
Fertil Steril. 1998 Sep;70(3):553-9. doi: 10.1016/s0015-0282(98)00243-x.
To investigate whether interleukin-1beta (IL-1beta) and interleukin-1alpha (IL-1alpha) affect the implantation rate of patients undergoing IVF-ET.
Follicular fluid and serum were obtained on the day of hCG administration, the day of oocyte retrieval, and the day of embryo transfer.
Cellular immunology laboratory in a research institute, a high technology IVF unit in a medical center, and a university hospital.
PATIENT(S): Thirty-three women who were undergoing IVF-ET.
MAIN OUTCOME MEASURE(S): IL-1beta and IL-1alpha were measured by specific ELISA and their levels were correlated with the implantation rate.
RESULT(S): Classification of IVF-ET patients according to their implantation rate revealed significantly higher amounts of follicular fluid IL-1beta in the implantation versus nonimplantation cycles (68.5+/-24.6 pg/mL versus 20.5+/-13.4 pg/mL); The difference between the level of IL-1alpha in the two groups was not statistically significant(11.6+/-5.1 pg/mL versus 7.3+/-1.9 pg/mL). In parallel, systemic FSH/hMG-dependent IL-1beta and IL-1alpha production was observed in implantation cycles but not in nonimplantation cycles. Statistically significant IL-1beta and IL-1alpha production was observed after administration of hCG.
CONCLUSION(S): Gonadotropins used during IVF-ET induce local and systemic production of IL-1beta and IL-1alpha. In addition, the implantation rate for IVF-ET patients who have detectable serum concentrations of IL-1beta and IL-1beta on the day of hCG administration could be higher than the rate for IVF-ET patients who do not have detectable concentrations of these cytokines.
研究白细胞介素-1β（IL-1β）和白细胞介素-1α（IL-1α）是否影响接受体外受精-胚胎移植（IVF-ET）患者的着床率。
在注射人绒毛膜促性腺激素（hCG）当天、取卵日和胚胎移植日获取卵泡液和血清。
一家研究所的细胞免疫学实验室、一家医疗中心的高科技体外受精单位和一家大学医院。
33名接受IVF-ET的女性。
通过特异性酶联免疫吸附测定（ELISA）法检测IL-1β和IL-1α，并将其水平与着床率进行关联。
根据着床率对IVF-ET患者进行分类显示，着床周期与未着床周期相比，卵泡液中IL-1β的含量显著更高（68.5±24.6 pg/mL对20.5±13.4 pg/mL）；两组中IL-1α水平的差异无统计学意义（11.6±5.1 pg/mL对7.3±1.9 pg/mL）。同时，在着床周期中观察到系统性促卵泡激素/人绝经期促性腺激素（FSH/hMG）依赖性IL-1β和IL-1α的产生，而在未着床周期中未观察到。注射hCG后观察到有统计学意义的IL-1β和IL-1α产生。
IVF-ET过程中使用的促性腺激素可诱导IL-1β和IL-1α的局部和全身产生。此外，在hCG给药当天血清中可检测到IL-1β和IL-1β浓度的IVF-ET患者的着床率可能高于未检测到这些细胞因子浓度的IVF-ET患者的着床率。
