慢生根瘤菌A1017中一种NAD-苹果酸酶的纯化与特性分析
Purification and characterization of an NAD-malic enzyme from Bradyrhizobium japonicum A1017.
作者信息
Chen F, Okabe Y, Osano K, Tajima S
机构信息
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Ikenobe 2393, Kita-gun, Kagawa 761-07, Japan.
出版信息
Appl Environ Microbiol. 1998 Oct;64(10):4073-5. doi: 10.1128/AEM.64.10.4073-4075.1998.
Abstract
An NAD-malic enzyme was purified to homogeneity from Bradyrhizobium japonicum A1017, and its molecular characteristics were surveyed. The enzyme exhibited native and subunit molecular masses of 388 and 85 kDa, respectively, suggesting that it exists as a homotetramer, and was activated by metabolic intermediates in glycolysis. The role of the enzyme in bacteroids' carbon metabolism is discussed.
摘要
从日本慢生根瘤菌A1017中纯化出一种烟酰胺腺嘌呤二核苷酸苹果酸酶并使其达到同质，同时对其分子特性进行了研究。该酶的天然分子量和亚基分子量分别为388 kDa和85 kDa，表明它以同四聚体形式存在，并被糖酵解中的代谢中间体激活。本文讨论了该酶在类菌体碳代谢中的作用。
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