用于前列腺癌和良性前列腺增生生长抑制研究的新型灵敏发现组织培养模型。

New sensitive discovery histoculture model for growth-inhibition studies in prostate cancer and BPH.

作者信息

Olbina G, Miljkovic D, Hoffman R M, Geller J

机构信息

AntiCancer, Inc., San Diego, California 92111, USA.

出版信息

Prostate. 1998 Oct 1;37(2):126-9. doi: 10.1002/(sici)1097-0045(19981001)37:2<126::aid-pros9>3.0.co;2-e.

DOI:10.1002/(sici)1097-0045(19981001)37:2<126::aid-pros9>3.0.co;2-e

PMID:9759707

Abstract

BACKGROUND

A new, total-immersion three-dimensional histoculture (TIH) method was developed to evaluate growth of tissue containing a mixture of benign prostate hyperplasia (BPH) and prostate cancer in vitro.

METHODS

Efficacy of inhibitors, such as genistein, was determined by measuring 3H-thymidine incorporation per microgram protein. Inhibitory effects obtained in TIH were compared to those in sponge-gel supported histoculture (SSH).

RESULTS

3H-thymidine incorporation was 2-5-fold higher in tissue cultured in TIH than in SSH. The average inhibition by genistein at a concentration of 18 JIM was 73% in TIH, vs. 31% in SSH. TIH also appeared to be more sensitive, since the lowest concentration of genistein that significantly inhibited growth of BPH mixed with prostate cancer tissue was 2.3 IJM, while in SSH the lowest concentration was 9.2 F,M. Although the within-assay coefficient of variation (CV) was similar for both TIH and SSH, the between-assay CV was better in TIH.

CONCLUSIONS

These data suggest that TIH can be used as a discovery model for screening and evaluating inhibitors of prostate tissue growth in vitro.

摘要

背景

开发了一种全新的全浸没式三维组织培养（TIH）方法，用于在体外评估含有良性前列腺增生（BPH）和前列腺癌混合物的组织生长情况。

方法

通过测量每微克蛋白质中3H-胸腺嘧啶核苷的掺入量来确定染料木黄酮等抑制剂的功效。将TIH中获得的抑制效果与海绵凝胶支持的组织培养（SSH）中的抑制效果进行比较。

结果

在TIH中培养的组织中，3H-胸腺嘧啶核苷的掺入量比在SSH中高2至5倍。染料木黄酮浓度为18 μM时，在TIH中的平均抑制率为73%，而在SSH中为31%。TIH似乎也更敏感，因为显著抑制BPH与前列腺癌组织混合生长的染料木黄酮最低浓度在TIH中为2.3 μM，而在SSH中为9.2 μM。尽管TIH和SSH的批内变异系数（CV）相似，但TIH的批间CV更好。