Thougaard A V, Høgdall C K, Kjaer S K, Blaakaer J, Jaliashvili I, Christiansen M
Department of Clinical Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Clin Chim Acta. 1998 Aug 10;276(1):19-34. doi: 10.1016/s0009-8981(98)00092-8.
The performance of two sandwich-type immunoassays for the determination of the tumour marker tetranectin using monoclonal antibodies Hyb 130-13 and 130-14 as catching layer was compared with the performance of a polyclonal assay. Sensitivities were 0.4-0.6 microg/l, and intra- and inter-assay coefficients of variation were < 10% in all assays. One-hundred-and-ten blood donors were examined, and women had higher concentrations of tetranectin in serum than men when measured with monoclonal assays (P < 0.05). In preoperative serum samples from 43 patients with ovarian cancer, tetranectin concentrations were reduced (P < 0.001), and the mean tetranectin concentration decreased with increasing FIGO stage of the patients (P < 0.05). In sera from patients with ovarian cancer, tetranectin concentrations were lower in the polyclonal assay than in the monoclonal assays. This could, hypothetically, be explained by ligand-binding or other conformational changes in tetranectin, influencing the antigenicity of the molecule.
比较了两种以单克隆抗体Hyb 130 - 13和130 - 14作为捕获层用于测定肿瘤标志物四连蛋白的夹心型免疫测定法与一种多克隆测定法的性能。所有测定法的灵敏度为0.4 - 0.6微克/升，批内和批间变异系数均<10%。对110名献血者进行了检测，使用单克隆测定法时，女性血清中四连蛋白的浓度高于男性（P < 0.05）。在43例卵巢癌患者的术前血清样本中，四连蛋白浓度降低（P < 0.001），并且患者的平均四连蛋白浓度随国际妇产科联盟（FIGO）分期的增加而降低（P < 0.05）。在卵巢癌患者的血清中，多克隆测定法测得的四连蛋白浓度低于单克隆测定法。从理论上讲，这可能是由于四连蛋白的配体结合或其他构象变化影响了该分子的抗原性所致。
