Dahl 大鼠组织去甲肾上腺素浓度与器官及发育相关的差异

Organ and development related difference in tissue norepinephrine concentrations in Dahl rats.

作者信息

Chen Q H, Nishida Y, Zhou M S, Murakami H, Morita H, Hosomi H, Kosaka H

机构信息

Second Department of Physiology, Kagawa Medical University, Japan.

出版信息

J Auton Nerv Syst. 1998 Jul 15;71(2-3):175-82. doi: 10.1016/s0165-1838(98)00082-4.

DOI:10.1016/s0165-1838(98)00082-4

PMID:9760054

Abstract

To determine organ and development related differences in tissue norepinephrine concentration (tNE) in Dahl salt-sensitive (S) and -resistant (R) rats, we measured the tNE of 16 organs, including the heart (left ventricle), kidney, cerebrum, brain stem, stomach, jejunum, ileum, colon, spleen, pancreas, liver, aorta, lung, bone, salivary gland, and muscle, at 1, 3, 5, 7, 9, 11 weeks old. Large differences were found in tNE among the organs of both S and R rats, ranging from 4.0 +/- 1.1 ng/g tissue (the bone of S rats) to 1234.8 +/- 32.5 ng/g tissue (the salivary gland of R rats). tNE in R rats increased development-dependently in 12 of 16 organs, but did not significantly change in the other three organs, and decreased in the bone. On the other hand, the development-dependent increase in tNE was suppressed in S rats, and the tNE values of S rats were significantly lower than those of R rats in 14 of 16 organs. To eliminate the baroreflexive effects on tNE, another group of 5-week-old S and R rats were subjected to sinoaortic denervation (SAD) or the sham operation. The tNE was measured in 10 organs in these animals at 9 weeks old. SAD did not alter the tNE in most of the organs in both S and R rats. There was no significant differences in mean arterial pressure (MAP) between S and R rats with baroreceptor intact at 9 weeks old. SAD slightly but significantly increased MAP in S rats, whereas not in R rats. There was no significant differences in plasma NE concentration (pNE) between S and R rats with the baroreceptor intact. SAD did not alter pNE in S or R rats. These results demonstrate that variations of the tNE were dependent on the organ and development. Many organs of S rats had lower tNE than those of R rats. The developmental-dependent increases in tNE in S rats were suppressed, compared with those in R rats. These tNE behaviors in S rats may not be related to blood pressure or baroreflex sensitivity, but might be involved in an abnormal sympathetic nerve activity.

摘要

为了确定 Dahl 盐敏感（S）和盐抵抗（R）大鼠组织去甲肾上腺素浓度（tNE）在器官及发育方面的差异，我们测量了 16 个器官的 tNE，这些器官包括心脏（左心室）、肾脏、大脑、脑干、胃、空肠、回肠、结肠、脾脏、胰腺、肝脏、主动脉、肺、骨骼、唾液腺和肌肉，测量时间点为 1、3、5、7、9、11 周龄。在 S 大鼠和 R 大鼠的器官中均发现 tNE 存在很大差异，范围从 4.0±1.1 ng/g 组织（S 大鼠的骨骼）到 1234.8±32.5 ng/g 组织（R 大鼠的唾液腺）。R 大鼠的 16 个器官中有 12 个器官的 tNE 随发育而增加，但其他三个器官没有显著变化，骨骼中的 tNE 则下降。另一方面，S 大鼠中 tNE 随发育的增加受到抑制，并且在 16 个器官中的 14 个器官中，S 大鼠的 tNE 值显著低于 R 大鼠。为了消除压力反射对 tNE 的影响，另一组 5 周龄的 S 大鼠和 R 大鼠接受了窦主动脉去神经支配（SAD）或假手术。在这些动物 9 周龄时测量了 10 个器官的 tNE。SAD 并未改变 S 大鼠和 R 大鼠大多数器官中的 tNE。9 周龄时压力感受器完整的 S 大鼠和 R 大鼠之间平均动脉压（MAP）没有显著差异。SAD 使 S 大鼠的 MAP 略有但显著升高，而 R 大鼠则没有。压力感受器完整的 S 大鼠和 R 大鼠之间血浆去甲肾上腺素浓度（pNE）没有显著差异。SAD 未改变 S 大鼠或 R 大鼠的 pNE。这些结果表明，tNE 的变化取决于器官和发育情况。S 大鼠的许多器官的 tNE 低于 R 大鼠。与 R 大鼠相比，S 大鼠中 tNE 随发育的增加受到抑制。S 大鼠中的这些 tNE 行为可能与血压或压力反射敏感性无关，但可能与异常的交感神经活动有关。