Walters D, Cowley T
Department of Plant Biology, Scottish Agricultural College, Auchincruive, Nr Ayr, UK.
Microbiol Res. 1998 Aug;153(2):179-84. doi: 10.1016/S0944-5013(98)80038-4.
Growth of the yeast Saccharomyces cerevisiae was unaffected by up to 24 h exposure to ethanol concentrations ranging from 1% to 9%, but was reduced following exposure to 12% ethanol. Concentrations of the polyamines putrescine, cadaverine and spermidine were not affected by a 24 h exposure to 12% ethanol, although there was a significant increase in spermine level. These changes were accompanied by significant increases in the activities of the polyamine biosynthetic enzymes ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) and in the flux of label from ornithine into the polyamines. Formation of the cadaverine derivatives aminopropylcadaverine and N,N-bis(3-aminopropyl)cadaverine was greatly increased in yeast exposed to 12% ethanol for 24 h, probably via the action of ODC, AdoMetDC and the aminopropyltransferases. Exposure to 12% ethanol also led to substantial reductions in the uptake of putrescine and spermidine and the amino acid methionine.
酿酒酵母（Saccharomyces cerevisiae）暴露于浓度为1%至9%的乙醇中长达24小时，其生长未受影响，但暴露于12%乙醇后生长受到抑制。多胺腐胺、尸胺和亚精胺的浓度在暴露于12%乙醇24小时后未受影响，不过精胺水平显著增加。这些变化伴随着多胺生物合成酶鸟氨酸脱羧酶（ODC）和S-腺苷甲硫氨酸脱羧酶（AdoMetDC）的活性显著增加，以及鸟氨酸中标记物向多胺的通量增加。在暴露于12%乙醇24小时的酵母中，尸胺衍生物氨丙基尸胺和N,N-双（3-氨丙基）尸胺的形成大幅增加，这可能是通过ODC、AdoMetDC和氨丙基转移酶的作用实现的。暴露于12%乙醇还导致腐胺、亚精胺和氨基酸甲硫氨酸的摄取大幅减少。
