Zhao W, Schafer R, Barnett J B
Department of Microbiology and Immunology, West Virginia University School of Medicine, Morgantown, USA.
J Toxicol Environ Health A. 1998 Sep 25;55(2):107-20. doi: 10.1080/009841098158548.
Numerous immunomodulatory effects are caused by propanil, an extensively used postemergent herbicide. The T-dependent antibody response is suppressed after exposure to propanil, raising the question of propanil's effect on T-helper-cell populations. In the present study, we show that the production of several T-cell cytokines is affected by propanil after in vivo or in vitro exposure. In vivo exposure to propanil caused the reduction of interleukin (IL)-2, IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and interferon (IFN)-gamma production in concanavalin A-stimulated spleen cell cultures established 2 d after exposure. IFN-gamma and GM-CSF production had recovered by d 4 postexposure; however, IL-2 and IL-6 levels continued to be depressed through d 7 postexposure. Continuous in vitro treatment of normal spleen cells with propanil decreased IL-2, IL-6, GM-CSF, and IFN-gamma production after concanavalin A activation. Pulsing normal spleen cell cultures with propanil for up to 8 h before T-cell activation resulted in reduced IL-6 but not IL-2 or IFN-gamma production. These data indicate that propanil can selectively inhibit spleen cell cytokine production, which could contribute to the immunomodulatory effects previously described.
敌稗是一种广泛使用的苗后除草剂，它具有多种免疫调节作用。接触敌稗后，T细胞依赖性抗体反应受到抑制，这引发了关于敌稗对辅助性T细胞群体影响的问题。在本研究中，我们发现，体内或体外接触敌稗后，几种T细胞细胞因子的产生会受到影响。体内接触敌稗会导致在接触后第2天建立的刀豆球蛋白A刺激的脾细胞培养物中白细胞介素（IL）-2、IL-6、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和干扰素（IFN）-γ的产生减少。接触后第4天，IFN-γ和GM-CSF的产生已恢复；然而，IL-2和IL-6水平在接触后第7天仍持续降低。用敌稗持续体外处理正常脾细胞会降低刀豆球蛋白A激活后IL-2、IL-6、GM-CSF和IFN-γ的产生。在T细胞激活前，用敌稗对正常脾细胞培养物进行长达8小时的脉冲处理会导致IL-6产生减少，但不会导致IL-2或IFN-γ产生减少。这些数据表明，敌稗可以选择性抑制脾细胞细胞因子的产生，这可能是先前所述免疫调节作用的原因。
