Schwarz M A, Raith K, Neubert R H
Martin Luther University Halle, Department of Pharmacy, Institute of Pharmaceutics and Biopharmaceutics, Saale, Germany.
Electrophoresis. 1998 Sep;19(12):2145-50. doi: 10.1002/elps.1150191218.
A practical approach for the characterization of pure micelles, and binary or ternary mixed micelles by capillary electrophoretic methods is presented. Interest is focused on the determination of mobility and composition of the micelles. The investigations are performed with bile salts, phosphatidylcholines and fatty acids. Pure bile salt micelles are characterized with the help of marking and displacement methods. Binary bile salt/phospholipid and ternary bile salt/phospholipid/fatty acid micelles are analyzed using capillary electrophoresis (CE) techniques with standard and improved UV detection, laser-induced fluorescence and electrospray ionization-mass spectrometry (ESI-MS). For both the binary and the ternary systems, only one stable mixed micellar phase is found with a high negative mobility.
本文提出了一种通过毛细管电泳方法表征纯胶束以及二元或三元混合胶束的实用方法。研究重点在于测定胶束的迁移率和组成。研究使用了胆汁盐、磷脂酰胆碱和脂肪酸进行。借助标记和置换方法对纯胆汁盐胶束进行了表征。采用具有标准和改进紫外检测、激光诱导荧光和电喷雾电离质谱（ESI-MS）的毛细管电泳（CE）技术对二元胆汁盐/磷脂和三元胆汁盐/磷脂/脂肪酸胶束进行了分析。对于二元和三元体系，均发现只有一个具有高负迁移率的稳定混合胶束相。
