Wright L M, Rizkallah P J, Wood S D, Reynolds C D
School of Biomolecular Sciences, Max Perutz Building, Liverpool John Moores University, Liverpool L3 3AF, England.
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):665-7. doi: 10.1107/s0907444997019859.
The monocot mannose-specific lectin, Scilla campanulata agglutinin (SCA), from bluebell bulbs has a strong affinity for alpha1,3- and alpha1,6-linked mannosyl residues. SCA has been co-crystallized with the trisaccharide alpha-D-mannopyranosyl-(1-->6)-alpha-D-mannopyranosyl-(1-->3)-alpha-D -mannopyranoside ¿alpha-D-Man-(1-->6)-[alpha-D-Man-(1-->3)]-alpha-D-Man¿, the core structure of biantennary N-linked oligosaccharides. Crystals of the complex were obtained by the hanging-drop vapour-diffusion technique. A complete data set to 2.5 A resolution has been collected at 100 K, using a MAR image-plate system at a synchrotron source, from crystals which belong to the space group C2 with unit-cell dimensions a = 99.38, b = 119.86, c = 77.10 A and beta = 105.56 degrees. Use of a CCD detector with cryo-cooled crystals improved the resolution to 2.3 A. A molecular replacement solution, with the 2.5 A data set, using the native SCA as a search model was obtained, with six subunits per asymmetric unit.
来自蓝铃花球茎的单子叶甘露糖特异性凝集素，绵枣儿凝集素（SCA），对α1,3-和α1,6-连接的甘露糖基残基具有很强的亲和力。SCA已与三糖α-D-甘露吡喃糖基-(1→6)-α-D-甘露吡喃糖基-(1→3)-α-D-甘露糖苷α-D-Man-(1→6)-[α-D-Man-(1→3)]-α-D-Man（双天线N-连接寡糖的核心结构）共结晶。通过悬滴气相扩散技术获得了复合物的晶体。在100 K下，使用同步辐射源的MAR成像板系统，从属于空间群C2、晶胞参数a = 99.38、b = 119.86、c = 77.10 Å且β = 105.56°的晶体中收集了分辨率为2.5 Å的完整数据集。使用带有低温冷却晶体的CCD探测器将分辨率提高到了2.3 Å。利用2.5 Å数据集，以天然SCA作为搜索模型，获得了分子置换解，每个不对称单元有六个亚基。
