Wright L M, Wood S D, Reynolds C D, Rizkallah P J, Allen A K
School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, England.
Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):90-2. doi: 10.1107/s0907444997007063.
Recent work has shown that Scilla campanulata agglutinin from bluebell bulbs has a strong affinity for alpha(1,3)- and alpha(1,6)-linked mannosyl residues and possesses moderate antiretroviral activity. This lectin has been crystallized by the hanging-drop method of vapour diffusion complexed with the disaccharide mannose-alpha1,6-D-mannose. The crystals are in the space group P21212 with unit-cell dimensions a = 70.63, b = 92.79 and c = 47.25 A, and with a dimer in the asymmetric unit. The crystals diffract X-rays to beyond 1.5 A resolution at 277 K and are stable in an X-ray beam. Data to 1.6 A resolution have been collected using a MAR image-plate system at a synchrotron source and the structure of the complex has been solved by the molecular replacement method.
最近的研究表明，来自蓝铃花球茎的绵枣儿凝集素对α(1,3)-和α(1,6)-连接的甘露糖基残基具有很强的亲和力，并具有中等的抗逆转录病毒活性。这种凝集素已通过气相扩散悬滴法与二糖甘露糖-α1,6-D-甘露糖复合结晶。晶体属于空间群P21212，晶胞参数a = 70.63、b = 92.79和c = 47.25 Å，不对称单元中有一个二聚体。晶体在277 K时能将X射线衍射到1.5 Å以上的分辨率，并且在X射线束中稳定。使用同步辐射源的MAR成像板系统收集了分辨率为1.6 Å的数据，并通过分子置换法解析了复合物的结构。
