Stillman L C, Gassman M L
Department of Biological Sciences, University of Illinois at Chicago Circle 60680, USA.
Anal Biochem. 1978 Nov;91(1):166-72. doi: 10.1016/0003-2697(78)90827-8.
A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether. After evaporation of the ether, the residue is dissolved in alkaline pyridine, and the protoheme concentration is estimated from a dithionite-reduced-minus-ferricyanide-oxidized spectrum. When compared to some other methods, this procedure gives consistently higher yields.
已开发出一种可重复估算植物组织中原卟啉含量的方法。将组织样品在80%丙酮中匀浆以去除色素和脂质；然后用2%盐酸丙酮溶液从组织残渣中提取原卟啉，并定量转移至乙醚中。乙醚蒸发后，残渣溶解于碱性吡啶中，通过连二亚硫酸盐还原减去铁氰化物氧化光谱估算原卟啉浓度。与其他一些方法相比，该方法的产量始终更高。
