通过与聚尿苷酸杂交来测定聚腺苷酸：由于氚标记的聚尿苷酸在三氯乙酸中不稳定而导致的误差来源。

Measurement of polyadenylic acid by hybridization with polyuridylic acid: a source of error due to the lability of tritiated polyuridylic acid in trichloroacetic acid.

作者信息

Williams D L, Klett H

机构信息

Department of Pharmacological Sciences, State University of New York at Stony Brook 11794, USA.

出版信息

Anal Biochem. 1978 Nov;91(1):173-9. doi: 10.1016/0003-2697(78)90828-x.

DOI:10.1016/0003-2697(78)90828-x

PMID:9762096

Abstract

During brief exposure to trichloroacetic acid at 0 degree C, significant amounts of tritiated polyuridylic acid are converted to acid-soluble products. The loss of polymeric radioactivity is dependent on both acid concentration and the time of exposure to acid. When trichloroacetic acid precipitation is used to recover the tritiated polyuridylic acid present in a hybrid with polyadenylic acid, significant underestimates of the hybridized radioactivity can occur because of the lability of tritiated polyuridylic acid. Conditions are given which minimize the lability and permit quantitative recovery of tritiated polyuridylic acid by trichloroacetic acid precipitation.

摘要

在0摄氏度下短暂暴露于三氯乙酸时，大量的氚标记聚尿苷酸会转化为酸溶性产物。聚合物放射性的损失取决于酸浓度和暴露于酸的时间。当使用三氯乙酸沉淀法回收与聚腺苷酸杂交体中存在的氚标记聚尿苷酸时，由于氚标记聚尿苷酸的不稳定性，可能会显著低估杂交放射性。文中给出了一些条件，这些条件可将不稳定性降至最低，并允许通过三氯乙酸沉淀法定量回收氚标记聚尿苷酸。