Coutts S M, Reid D M
Department of Biochemistry and Drug Disposition, USV Pharmaceutical Corporation, Tuckahoe, New York 10707, USA.
Anal Biochem. 1978 Dec;91(2):446-50. doi: 10.1016/0003-2697(78)90530-4.
A method utilizing high-voltage electrophoresis on paper is described whereby a pentapeptide (Asp-Ser-Asp-Pro-Arg) labeled with Bolton-Hunter reagent is separated from hydrolyzed reagent and unreacted peptide and is recovered from the electrophoretogram in high yield. The general applicability to other peptides is discussed.
本文描述了一种利用纸高压电泳的方法，通过该方法，用博尔顿-亨特试剂标记的五肽（天冬氨酸-丝氨酸-天冬氨酸-脯氨酸-精氨酸）可与水解试剂和未反应的肽分离，并以高产率从电泳图谱中回收。文中还讨论了该方法对其他肽的普遍适用性。
