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用SP葡聚糖凝胶色谱法测定S-腺苷-L-甲硫氨酸水平。

Measurement of S-adenosyl-L-methionine levels by SP Sephadex chromatography.

作者信息

Glazer R I, Peale A L

机构信息

Laboratory of Medicinal Chemistry and Biology, National Cancer Institute, Bethesda, Maryland 20014, USA.

出版信息

Anal Biochem. 1978 Dec;91(2):516-20. doi: 10.1016/0003-2697(78)90538-9.

DOI:10.1016/0003-2697(78)90538-9
PMID:9762138
Abstract

Cation-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl-3H]adenosyl-L-methionine synthesized by L1210 cells in vitro. Separation of S-[methyl-3H]adenosyl-L-methionine from [methyl-3H]methionine and other metabolites was achieved by stepwise elution with varying concentrations of HCl.

摘要

采用磺酸丙基葡聚糖凝胶阳离子交换色谱法来测定L1210细胞在体外合成的S-[甲基-³H]腺苷-L-甲硫氨酸的水平。通过用不同浓度的盐酸进行分步洗脱，实现了将S-[甲基-³H]腺苷-L-甲硫氨酸与[甲基-³H]甲硫氨酸及其他代谢产物分离。

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