Zappia V, Galletti P, Porcelli M, Manna C, Ragione F D
J Chromatogr. 1980 Mar 7;189(3):399-405. doi: 10.1016/s0021-9673(00)80319-2.
A rapid, sensitive and specific high-performance liquid chromatographic method for the simultaneous separation of natural adenosyl-sulphur compounds has been developed. The compounds were separated by using the strong cation-exchange resin Partisil 10 SCX with isocratic elution. The adenosyl-compounds were monitored by an ultraviolet detector operating at 254 nm. Sensitivity was greater than 50 pmoles for all compounds tested and standard curves were found to be linear for concentrations of up to 50 nmoles. The method can be applied to biological samples for the estimation of S-adenosyl-L-methionine,S-adenosyl-L-homocysteine and S-adenosyl-(5')-3-methyl-thiopropylamine and for measurement of enzyme activities involving the above-mentioned compounds.
已开发出一种快速、灵敏且特异的高效液相色谱法，用于同时分离天然腺苷 - 硫化合物。使用强阳离子交换树脂Partisil 10 SCX通过等度洗脱分离这些化合物。通过在254 nm运行的紫外检测器监测腺苷化合物。对所有测试化合物的灵敏度均大于50皮摩尔，并且发现浓度高达50纳摩尔时标准曲线呈线性。该方法可应用于生物样品，用于估算S - 腺苷 - L - 甲硫氨酸、S - 腺苷 - L - 高半胱氨酸和S - 腺苷 - (5') - 3 - 甲基硫丙胺，以及测量涉及上述化合物的酶活性。
