一种使用[32P]乙酰磷酸和乙酸激酶合成[γ-32P]核苷三磷酸的简单方法。
A simple method for synthesizing [gamma-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase.
作者信息
Bauer P I, Várady G
机构信息
Second Institute of Biochemistry, Semmelweis University, Medical School, Budapest, Hungary.
出版信息
Anal Biochem. 1978 Dec;91(2):613-7. doi: 10.1016/0003-2697(78)90547-x.
PMID:9762147
Abstract
A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a final purification step. The entire procedure is performed 8 h or less.
摘要
本文描述了一种用于合成γ-32P标记的核糖或脱氧核糖核苷三磷酸的简单、快速且廉价的方法。该方法包括[32P]乙酰磷酸的化学合成，随后使用乙酸激酶（EC 2.7.2.1）对核苷二磷酸进行磷酸化以及最后的纯化步骤。整个过程在8小时或更短时间内完成。
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