Mingers A M
Eur J Pediatr. 1976 Oct 1;123(3):199-213. doi: 10.1007/BF00452097.
Factor VIII exchange test experiments with hemophilia A plasmas were performed to find out how the results were affected by submitting plasmas and plasma mixtures to different incubation periods at 37 degrees C, heat precipitation, and ether extraction. The experiments led to the following results: 1. In plasma mixtures, frequently higher factor VIII activities are found than can be expected from the single activities of the used plasmas. Activity increases are factor-specific. 2. The component to be activated is in the hemophilic plasma; the activity-increasing agent is in the normal plasma. These results lead to the hypothesis that hemophilia A patients have sufficient quantities of inactive factor VIII, but it stays inactive for lack of the necessary activator. In normal plasma the activator is in balance or surplus to the inactive factor. By adding normal plasma, thus supplying free activator, the inactive factor VIII of hemophilia A plasma can, under suitable conditions, be developed into active factor VIII.
进行了甲型血友病血浆的因子VIII交换试验，以探究将血浆及血浆混合物在37℃下置于不同孵育时间、热沉淀及乙醚萃取后，结果会受到怎样的影响。实验得出以下结果：1. 在血浆混合物中，经常会发现因子VIII活性高于根据所用血浆的单一活性所预期的值。活性增加具有因子特异性。2. 待激活的成分存在于血友病血浆中；活性增强剂存在于正常血浆中。这些结果引出一个假设，即甲型血友病患者有足够量的无活性因子VIII，但由于缺乏必要的激活剂而保持无活性状态。在正常血浆中，激活剂与无活性因子处于平衡或过剩状态。通过添加正常血浆从而提供游离激活剂，在合适条件下，甲型血友病血浆中的无活性因子VIII可转化为活性因子VIII。
