牛片形吸虫感染诊断的斑点酶联免疫吸附测定法的设计与评估
Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle.
作者信息
Arjmand Jafar, Esmaeilnejad Bijan, Razi Jalali Mohammad Hossein, Ghorbanpoor Masoud, Abtahi Froushani Seyyed Meysam
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Department of Pathobiology, Faculty of Veterinary Medicine, Ahvaz University, Ahvaz, Iran;
出版信息
Vet Res Forum. 2014 Spring;5(2):141-4.
Fasciolosis is a disease caused by liver fluck of the genus of Fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion-secretory (ES Ag) and Crude (Cr Ag) antigens of Fasciola was described for diagnosis of fasciolosis in cattle. For this purpose, the sera specimens of slaughtered cattle were taken and examined for Fasciola infection. Sera from two groups of cattle, one infected with Fasciola (n = 60) and the other non-infected with Fasciola (n = 60), were used in the Dot- ELISA test. All sera were tested and evaluated. Except specificity, other parameters such as, sensitivity, accuracy, positive and negative predictive values of Dot- ELISA with ES Ag were better than those of Dot- ELISA with Cr Ag. In conclusion, excretory-secretory antigen dependent Dot-ELISA can be used as a reliable sero-diagnostic test for Fasciola infection in cattle.
肝片吸虫病是由肝片吸虫属的肝吸虫引起的一种疾病。长期以来，由于粪便学诊断方法的敏感性较低，肝片吸虫病的诊断一直具有挑战性。在本研究中，描述了一种用于诊断牛肝片吸虫病的内部斑点酶联免疫吸附测定（Dot-ELISA）方法；该方法使用肝片吸虫的排泄分泌（ES Ag）抗原和粗抗原（Cr Ag）。为此，采集了屠宰牛的血清样本，并检测是否感染肝片吸虫。两组牛的血清，一组感染肝片吸虫（n = 60），另一组未感染肝片吸虫（n = 60），用于斑点酶联免疫吸附测定试验。对所有血清进行了检测和评估。除特异性外，斑点酶联免疫吸附测定法检测ES Ag的其他参数，如敏感性、准确性、阳性和阴性预测值，均优于检测Cr Ag的斑点酶联免疫吸附测定法。总之，基于排泄分泌抗原的斑点酶联免疫吸附测定法可作为牛肝片吸虫感染的可靠血清学诊断试验。
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