阿尔茨海默病大脑中蛋白质硝基酪氨酸和二酪氨酸的电化学分析表明存在区域特异性积累。
Electrochemical analysis of protein nitrotyrosine and dityrosine in the Alzheimer brain indicates region-specific accumulation.
作者信息
Hensley K, Maidt M L, Yu Z, Sang H, Markesbery W R, Floyd R A
机构信息
Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA.
出版信息
J Neurosci. 1998 Oct 15;18(20):8126-32. doi: 10.1523/JNEUROSCI.18-20-08126.1998.
Abstract
HPLC with electrochemical array detection (HPLC-ECD) was used to quantify 3,3'-dityrosine (diTyr) and 3-nitrotyrosine (3-NO2-Tyr) in four regions of the human brain that are differentially affected in Alzheimer's disease (AD). DiTyr and 3-NO2-Tyr levels were elevated consistently in the hippocampus and neocortical regions of the AD brain and in ventricular cerebrospinal fluid (VF), reaching quantities five- to eightfold greater than mean concentrations in brain and VF of cognitively normal subjects. Uric acid, a proposed peroxynitrite scavenger, was decreased globally in the AD brain and VF. The results suggest that AD pathogenesis may involve the activation of oxidant-producing inflammatory enzyme systems, including nitric oxide synthase.
摘要
采用高效液相色谱-电化学阵列检测法（HPLC-ECD）对阿尔茨海默病（AD）中受不同影响的人类大脑四个区域的3,3'-二酪氨酸（二酪氨酸）和3-硝基酪氨酸（3-NO2-酪氨酸）进行定量分析。AD大脑的海马体和新皮质区域以及脑室脑脊液（VF）中的二酪氨酸和3-NO2-酪氨酸水平持续升高，其含量比认知正常受试者大脑和VF中的平均浓度高出五至八倍。尿酸是一种推测的过氧亚硝酸盐清除剂，在AD大脑和VF中整体减少。结果表明，AD的发病机制可能涉及包括一氧化氮合酶在内的产氧化剂炎症酶系统的激活。
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