LEC大鼠细胞对拓扑异构酶II抑制剂椭圆玫瑰树碱具有更高的敏感性。

Higher sensitivity in LEC rat cells to a topoisomerase II inhibitor, ellipticine.

作者信息

Hayashi M, Ichikawa Y, Arai S, Endoh D, Okui T

机构信息

Department of Veterinary Radiology, Faculty of Veteriniary Medicine, Rakuno Gakuen University, Ebetsu, Japan.

出版信息

J Vet Med Sci. 1998 Aug;60(8):969-71. doi: 10.1292/jvms.60.969.

DOI:10.1292/jvms.60.969

PMID:9764413

Abstract

A concentration of ellipticine, an inhibitor of topoisomerase II, required to reduce cell survival to 37% (D37) is used as an index to compare the cellular sensitivity. D37 values of LEC and WKAH rat cells were 1.2 and 2.2 microM, respectively. Thus, LEC rat cells were approximately 1.8-fold more sensitive than WKAH rat cells to ellipticine. There was no significant difference between the topoisomerase II activities in nuclear extracts of LEC and WKAH rat cells. These results suggested that the high sensitivity of LEC rat cells to ellipticine is not associated with the level of topoisomerase II activity.

摘要

拓扑异构酶II抑制剂椭圆玫瑰树碱使细胞存活率降至37%（D37）所需的浓度，被用作比较细胞敏感性的指标。LEC和WKAH大鼠细胞的D37值分别为1.2和2.2微摩尔。因此，LEC大鼠细胞对椭圆玫瑰树碱的敏感性约为WKAH大鼠细胞的1.8倍。LEC和WKAH大鼠细胞核提取物中的拓扑异构酶II活性之间没有显著差异。这些结果表明，LEC大鼠细胞对椭圆玫瑰树碱的高敏感性与拓扑异构酶II活性水平无关。