Andjus R K, Damjanović I, Gacić Z, Konjević D J, Andjus P R
Biophysical Laboratory, Center for Multidisciplinary Studies, University of Belgrade, Yugoslavia.
Vis Neurosci. 1998 Sep-Oct;15(5):923-30. doi: 10.1017/s0952523898155189.
Although differences in visual pigments between developmental stages of the European eel are well known, the expected differences in spectral sensitivity have not been demonstrated at the electrophysiological level. In fact, one past electroretinographic study led to the conclusion that in eels there is no change in scotopic sensitivity, with increasing sexual maturity. In the present experiments, electroretinograms (ERGs) were recorded from in situ eyecups of immobilized eels Anguilla anguilla (L.) caught in coastal running waters. It was shown that the ERG b-wave is as good an indicator of spectral sensitivity as the unmasked late receptor potential (LRP) which directly reflects the responsiveness of photoreceptors. Complete spectral-sensitivity curves, based on b-wave thresholds and on thresholds of LRP subsequently isolated by means of sodium iodate, have been obtained in the same eel. Using fitted amplitude-log intensity functions for threshold calculation, and two models for computer-assisted fitting of spectral-sensitivity curves, significant differences in lambda max were found between yellow and silver developmental stages of the eel, identified by ocular index measurements.
尽管欧洲鳗鲡发育阶段之间视觉色素的差异已广为人知，但光谱敏感性方面预期的差异尚未在电生理水平上得到证实。事实上，过去一项视网膜电图研究得出结论，随着性成熟度增加，鳗鲡的暗视觉敏感性并无变化。在本实验中，从捕获于沿海流水区域的固定欧洲鳗鲡（Anguilla anguilla, L.）的原位眼杯中记录视网膜电图（ERG）。结果表明，ERG的b波与直接反映光感受器反应性的未掩盖晚期感受器电位（LRP）一样，都是光谱敏感性的良好指标。基于b波阈值以及随后通过碘酸钠分离出的LRP阈值，在同一条鳗鲡中获得了完整的光谱敏感性曲线。使用拟合的振幅-对数强度函数进行阈值计算，并采用两种计算机辅助拟合光谱敏感性曲线的模型，通过眼指数测量确定的鳗鲡黄色和银色发育阶段之间，发现最大波长（lambda max）存在显著差异。
