Zahn M, Seeger S
Institut für Analytische Chemie, Chemo- und Biosensorik, University of Regensburg, Germany.
Cell Mol Biol (Noisy-le-grand). 1998 Jul;44(5):747-61.
Current applications of optical tweezers in pharmacology are presented. The manufacture of cellular biosensor arrays employing optical tweezers is reviewed. Using this technique, a new approach to cellular drug screening, based on single cells patterned with the laser tweezers was introduced. Specific stimulation of different immobilized, viable cells could be shown simultaneously. Furthermore, the usefulness of optical tweezers for analyzing the interactions of ligands with cellular membrane receptors is demonstrated. The laser tweezers could successfully be used to compare neuron interactions with glycoproteins of the extracellular matrix by applying the optical tweezers as picotensometer. The forces of interactions between polystyrene beads coated with different proteins of the extracellular matrix and the cell membrane receptors of cerebellar neurons from postnatal day 6 (P6) mice were measured. When antibodies to the extracellular matrix proteins were added, forces were significantly reduced for the corresponding antigens but not for the other glycoproteins. This proved the specificity of the measured interactions. Information regarding the receptor anchorage of tenascin-C could be deduced.
介绍了光镊在药理学中的当前应用。综述了采用光镊制造细胞生物传感器阵列的情况。利用该技术，引入了一种基于用激光镊图案化的单细胞进行细胞药物筛选的新方法。可以同时显示对不同固定化活细胞的特异性刺激。此外，还证明了光镊在分析配体与细胞膜受体相互作用方面的有用性。通过将光镊用作皮克张力计，激光镊可成功用于比较神经元与细胞外基质糖蛋白的相互作用。测量了涂有细胞外基质不同蛋白质的聚苯乙烯珠与出生后第6天（P6）小鼠小脑神经元细胞膜受体之间的相互作用力。当加入针对细胞外基质蛋白质的抗体时，相应抗原的作用力显著降低，但其他糖蛋白的作用力未降低。这证明了所测量相互作用的特异性。可以推断出关于腱生蛋白-C受体锚定的信息。
