Arai Fumihito, Ng Chinaik, Maruyama Hisataka, Ichikawa Akihiko, El-Shimy Haitham, Fukuda Toshio
Department of Micro-Nano Systems Engineering, Nagoya University, Chikusa-ku, Nagoya, 464-8603, Japan.
Lab Chip. 2005 Dec;5(12):1399-403. doi: 10.1039/b502546j. Epub 2005 Oct 17.
A novel approach appropriate for rapid separation and immobilization of a single cell by concomitantly utilizing laser manipulation and locally thermosensitive hydrogelation is proposed in this paper. We employed a single laser beam as optical tweezers for separating a target cell and locating it adjacent to a fabricated, transparent micro heater. Simultaneously, the target cell is immobilized or partially entrapped by heating the thermosensitive hydrogel with the micro heater. The state of the thermosensitive hydrogel can be switched from sol to gel and gel to sol by controlling the temperature through heating and cooling by the micro heater. After other unwanted cells are removed by the high-speed cleaning flow in the microchannel, the entrapped cell is successfully isolated. It is possible to collect the immobilized target cell for analysis or culture by switching off the micro heater and releasing the cell from the entrapment. We demonstrated that the proposed approach is feasible for rapid manipulation, immobilization, cleaning, isolation and extraction of a single cell. The experimental results are shown here.
本文提出了一种通过同时利用激光操纵和局部热敏水凝胶化来快速分离和固定单个细胞的新方法。我们使用单束激光作为光镊来分离目标细胞，并将其定位在制造的透明微型加热器附近。同时，通过微型加热器加热热敏水凝胶，使目标细胞固定或部分被捕获。通过微型加热器加热和冷却来控制温度，可使热敏水凝胶的状态从溶胶转变为凝胶，再从凝胶转变为溶胶。在通过微通道中的高速清洗流去除其他不需要的细胞后，被捕获的细胞成功分离。通过关闭微型加热器并将细胞从捕获状态释放出来，可以收集固定的目标细胞进行分析或培养。我们证明了所提出的方法对于单个细胞的快速操纵、固定、清洗、分离和提取是可行的。实验结果如下所示。
