de Courten Myers G M, Kleinholz M, Wagner K R, Myers R E
Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, OH 45267-0529, USA.
J Neurosci Methods. 1998 Sep 1;83(2):151-7. doi: 10.1016/s0165-0270(98)00073-9.
We presently examine the relation between histologic infarct size and neurologic deficit as endpoints and seek to clarify their sensitivity in defining stroke outcome. Neurologic deficits of 76 cats subjected to middle cerebral artery occlusion were assessed daily and correlated with the corresponding infarct sizes determined morphometrically after 2 weeks' survival. A five-item neurologic deficit score included the time elapsed until hemiparesis, and forced circling resolved (if ever), presence of impaired placing reactions and time elapsed until able to stand and being alert. We then evaluated the two endpoints' statistical powers to detect group differences using two sets of comparison groups. The neurologic deficit score correlated well with infarct size (r = 0.76, p < 0.001) and each of the individual deficit score components named above, in turn, correlated with decreasing power with infarct size. Even so, the number of study subjects required to achieve the same level of statistical significance in assessing group differences was two-fold greater when using the neurologic deficit than the infarct size data: Group sizes of eight and five animals were sufficient for significant infarct size differences while the groups needed be expanded to 15 and 10 animals to similarly achieve significant neurologic score differences. Thus, infarct size emerges as a more sensitive measure of stroke outcome than does the assessment of neurologic deficits.
我们目前以组织学梗死面积和神经功能缺损作为终点来研究它们之间的关系，并试图阐明它们在定义卒中结局方面的敏感性。对76只大脑中动脉闭塞的猫的神经功能缺损进行每日评估，并将其与存活2周后通过形态学测定确定的相应梗死面积相关联。一个五项神经功能缺损评分包括直到出现偏瘫和强迫转圈消失（如果有的话）所经过的时间、放置反应受损的情况以及直到能够站立和保持警觉所经过的时间。然后，我们使用两组比较组评估这两个终点检测组间差异的统计功效。神经功能缺损评分与梗死面积相关性良好（r = 0.76，p < 0.001），并且上述每个单独的缺损评分成分依次与梗死面积的检测功效降低相关。即便如此，在评估组间差异时，要达到相同水平的统计学显著性，使用神经功能缺损数据所需的研究对象数量是使用梗死面积数据时的两倍：8只和5只动物的组规模足以检测出梗死面积的显著差异，而组规模需要扩大到15只和10只动物才能同样检测出神经功能评分的显著差异。因此，梗死面积比神经功能缺损评估更能敏感地衡量卒中结局。
