Corrèges P, Bugnard E, Millerin C, Masiero A, Andrivet J P, Bloc A, Dunant Y
NEUROPHARMA, Centre Interuniversitaire de Recherche et d'Enseignement, Archamps, France.
J Neurosci Methods. 1998 Sep 1;83(2):177-84. doi: 10.1016/s0165-0270(98)00079-x.
Most of the parameters recorded in electrophysiology are strongly temperature dependent. In order to control temperature fluctuations we have built a system that ensures an accurate thermoregulation of the recording chamber. Temperature of physiological preparations can be changed relatively quickly (about 8 degrees C/min) and with a good accuracy (+/- 0.5 degrees C) without inducing thermal oscillations. Contrary to other thermoregulating devices, the temperature regulation is not carried out through the perfused medium but directly at the bottom of the chamber where a 3-cm2 Peltier element has been placed. The element is driven by a dedicated electronic device which controls the amount and the direction of the current flowing across the Peltier thermocouple. All construction details and the appropriate electrical circuits are provided. Using this home-made device, the steady-state chamber temperature could be precisely monitored with a resolution of +/- 0.1 degrees C in a range of 0-40 degrees C. This set-up was tested in experiments designed to evaluate the temperature dependence of synaptic transmission in the Torpedo nerve electroplate synapses and of calcium currents recorded from isolated nerve cells. This low-cost method is suitable for a wide range of applications.
电生理学中记录的大多数参数都强烈依赖于温度。为了控制温度波动，我们构建了一个系统，该系统可确保对记录室进行精确的温度调节。生理制剂的温度可以相对快速地改变（约8摄氏度/分钟），并且精度良好（±0.5摄氏度），而不会引起热振荡。与其他温度调节装置不同，温度调节不是通过灌注介质进行，而是直接在放置了3平方厘米珀耳帖元件的腔室底部进行。该元件由一个专用电子设备驱动，该设备控制流过珀耳帖热电偶的电流的大小和方向。文中提供了所有结构细节和合适的电路。使用这个自制设备，可以在0至40摄氏度的范围内以±0.1摄氏度的分辨率精确监测腔室的稳态温度。该装置在旨在评估电鳐神经电板突触中突触传递的温度依赖性以及从分离的神经细胞记录的钙电流的温度依赖性的实验中进行了测试。这种低成本方法适用于广泛的应用。
