Asteriti Sabrina, Cangiano Lorenzo
Dept. of Translational Research, University of Pisa, Pisa, Italy.
Dept. of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
HardwareX. 2020 Oct;8:e00155. doi: 10.1016/j.ohx.2020.e00155.
Effective temperature control is crucial in many studies of isolated biological tissues, with preparations often requiring specialized holding chambers. In these situations, the design flexibility and optimizations offered by a custom made temperature controller may be preferable over a commercial model. We present a versatile controller for heating and cooling applications, providing simple step-by-step instructions to mathematically model your specific system and optimize controller parameters. The apparatus uses analog components and linear stages to simplify circuit comprehension and customization, achieving fast transitions with small static errors and overshoots over a wide range of temperatures without readjustment. A fully featured rackable enclosure is complemented by two temperature probes based on the LMT70A linear microchip sensor (for the control loop and for bath monitoring). BNC outputs provide scaled probe signals for continuous temperature data acquisition. The maximum achievable power output of the controller is -23.5 W/+22.0 W (-4.7 V/+4.4 V, ±5.0 A), sufficient to bring a well designed holder for standard 35 mm chambers from 23 °C up to 37 °C in ~1 min and down to 3 °C in ~4 min. Any biologist with some technical prowess should be able to follow our instructions from modeling to assembly and calibration.
在许多对离体生物组织的研究中，有效的温度控制至关重要，相关实验准备通常需要专门的保温腔室。在这些情况下，定制温度控制器所提供的设计灵活性和优化功能可能比商业型号更具优势。我们展示了一种适用于加热和冷却应用的多功能控制器，并提供简单的逐步说明，以便对您的特定系统进行数学建模并优化控制器参数。该装置使用模拟组件和线性平台来简化电路理解和定制，在无需重新调整的情况下，能在很宽的温度范围内实现快速转换，且静态误差和过冲都很小。一个功能齐全的可上架机箱配有两个基于LMT70A线性微芯片传感器的温度探头（一个用于控制回路，一个用于浴槽监测）。BNC输出提供经过缩放的探头信号，用于连续温度数据采集。该控制器的最大可实现功率输出为-23.5 W / +22.0 W（-4.7 V / +4.4 V，±5.0 A），足以在约1分钟内将精心设计的标准35毫米腔室的夹具从23°C加热到37°C，并在约4分钟内冷却到3°C。任何具备一定技术能力的生物学家都应该能够按照我们的说明进行建模、组装和校准。
