HIV-1反式激活因子Tat的细胞共激活因子Tat-SF1是一种通用转录延伸因子。
The HIV-1 Tat cellular coactivator Tat-SF1 is a general transcription elongation factor.
作者信息
Li X Y, Green M R
机构信息
Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA.
出版信息
Genes Dev. 1998 Oct 1;12(19):2992-6. doi: 10.1101/gad.12.19.2992.
Abstract
The human immunodeficiency virus type 1 (HIV-1) Tat protein strongly and specifically stimulates transcription elongation from the HIV-1 LTR and provides an important in vitro model system to study this process. Here we use protein-affinity chromatography to identify cellular factors involved in transcription elongation. A Tat-affinity column bound one transcription factor, Tat-SF1, efficiently and selectively. Tat-SF1 was identified originally as a Tat-specific coactivator, but we show it is a general transcription elongation factor. Our results also reveal the existence of an ATP-inactivatable general elongation factor (AIEF) required for Tat-SF1 activity and for which Tat can substitute functionally.
摘要
1型人类免疫缺陷病毒（HIV-1）的Tat蛋白能强烈且特异性地刺激HIV-1长末端重复序列（LTR）的转录延伸，并为研究该过程提供了一个重要的体外模型系统。在此，我们利用蛋白质亲和色谱法来鉴定参与转录延伸的细胞因子。一个Tat亲和柱能高效且选择性地结合一种转录因子，即Tat-SF1。Tat-SF1最初被鉴定为一种Tat特异性共激活因子，但我们发现它是一种通用的转录延伸因子。我们的结果还揭示了存在一种Tat-SF1活性所需的、可被ATP灭活的通用延伸因子（AIEF），并且Tat在功能上可以替代该因子。
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本文引用的文献
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EMBO J. 1998 Jul 1;17(13):3681-91. doi: 10.1093/emboj/17.13.3681.
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PITALRE, the catalytic subunit of TAK, is required for human immunodeficiency virus Tat transactivation in vivo.TAK的催化亚基PITALRE在体内是人类免疫缺陷病毒Tat反式激活所必需的。
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Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation.酵母转录因子SPT5的人类同源物在HIV-1 Tat激活中的作用。
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A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA.一种新型的与CDK9相关的C型细胞周期蛋白直接与HIV-1反式激活因子（Tat）相互作用，并介导其与TAR RNA的高亲和力、环特异性结合。
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