大鼠周期同源物（rPer2）mRNA的多组织昼夜节律表达受哺乳动物昼夜节律时钟——大脑中的视交叉上核调控。

Multitissue circadian expression of rat period homolog (rPer2) mRNA is governed by the mammalian circadian clock, the suprachiasmatic nucleus in the brain.

作者信息

Sakamoto K, Nagase T, Fukui H, Horikawa K, Okada T, Tanaka H, Sato K, Miyake Y, Ohara O, Kako K, Ishida N

机构信息

Ishida Group of Clock Gene, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI, Higashi 1-1, Tsukuba, Ibaraki 305-8566, Japan.

出版信息

J Biol Chem. 1998 Oct 16;273(42):27039-42. doi: 10.1074/jbc.273.42.27039.

DOI:10.1074/jbc.273.42.27039

PMID:9765215

Abstract

The period (per) gene, controlling circadian rhythms in Drosophila, is expressed throughout the body in a circadian manner. A homolog of Drosophila per was isolated from rat and designated as rPer2. The rPER2 protein showed 39 and 95% amino acid identity with mPER1 and mPER2 (mouse homologs of per) proteins, respectively. A robust circadian fluctuation of rPer2 mRNA expression was discovered not only in the suprachiasmatic nucleus (SCN) of the hypothalamus but also in other tissues including eye, brain, heart, lung, spleen, liver, and kidney. Furthermore, the peripheral circadian expression of rPer2 mRNA was abolished in SCN-lesioned rats that showed behavioral arrhythmicity. These findings suggest that the multitissue circadian expression of rPer2 mRNA was governed by the mammalian brain clock SCN and also suggest that the rPer2 gene was involved in the circadian rhythm of locomotor behavior in mammals.

摘要

控制果蝇昼夜节律的周期（per）基因，以昼夜节律的方式在全身表达。从大鼠中分离出果蝇per的一个同源物，并将其命名为rPer2。rPER2蛋白与mPER1和mPER2（per的小鼠同源物）蛋白的氨基酸同一性分别为39%和95%。不仅在下丘脑的视交叉上核（SCN）中发现了rPer2 mRNA表达的强烈昼夜波动，在包括眼睛、大脑、心脏、肺、脾脏、肝脏和肾脏在内的其他组织中也发现了这种波动。此外，在表现出行为节律紊乱的SCN损伤大鼠中，rPer2 mRNA的外周昼夜节律表达被消除。这些发现表明，rPer2 mRNA在多组织中的昼夜节律表达受哺乳动物脑时钟SCN的调控，也表明rPer2基因参与了哺乳动物运动行为的昼夜节律。