Franti M, Aubin J T, Poirel L, Gautheret-Dejean A, Candotti D, Huraux J M, Agut H
Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpêtrière, 75651 Paris Cedex 13, France.
J Virol. 1998 Nov;72(11):8725-30. doi: 10.1128/JVI.72.11.8725-8730.1998.
As for other herpesviruses, glycoprotein B (gB) of human herpesvirus 7 (HHV-7) is believed to play a major role in virus infection and as a target of the host immunogenic response. Using nested PCR, we amplified the whole HHV-7 gB gene from 108 human peripheral blood mononuclear cell samples and studied its variability. By means of restriction fragment length polymorphism (RFLP) analysis, three distinct patterns, designated I, II, and III, were defined and detected at frequencies of 93, 5, and 2%, respectively. Determination of the nucleotide sequence allowed us to recognize five critical positions in the gB gene with six specific combinations of point changes at these positions. These combinations were gB alleles A, B, C, D, E, and F. Alleles D and E corresponded to RFLP patterns II and III, respectively, while the other four alleles corresponded to RFLP pattern I. Identical gB alleles were detected in serial samples as well as in paired samples of blood and saliva from the same individuals, except for one case. In contrast, the distribution of gB alleles differed according to the geographical origin of the human samples: C was the most frequent allele in both African and Caribbean samples, whereas F was the most frequent allele in European ones. Although none of the allele-specific nucleotide changes induced any modification at the protein level, the definition of gB alleles provided convenient viral markers for the study of both HHV-7 infections and human population genetics.
至于其他疱疹病毒，人们认为人类疱疹病毒7型（HHV - 7）的糖蛋白B（gB）在病毒感染中起主要作用，并作为宿主免疫原性反应的靶点。我们使用巢式聚合酶链反应（PCR）从108份人类外周血单个核细胞样本中扩增出完整的HHV - 7 gB基因，并研究其变异性。通过限制性片段长度多态性（RFLP）分析，定义并检测到三种不同的模式，分别命名为I、II和III，其频率分别为93%、5%和2%。核苷酸序列的测定使我们能够识别gB基因中的五个关键位置，这些位置存在六种特定的点突变组合。这些组合就是gB等位基因A、B、C、D、E和F。等位基因D和E分别对应RFLP模式II和III，而其他四个等位基因对应RFLP模式I。除了一个病例外，在连续样本以及同一个体的血液和唾液配对样本中检测到相同的gB等位基因。相比之下，gB等位基因的分布因人类样本的地理来源而异：在非洲和加勒比海样本中，C是最常见的等位基因，而在欧洲样本中，F是最常见的等位基因。尽管等位基因特异性核苷酸变化在蛋白质水平上未引起任何修饰，但gB等位基因的定义为研究HHV - 7感染和人类群体遗传学提供了便利的病毒标记。
