牛病毒性腹泻病毒非结构蛋白5B区域内RNA依赖性RNA聚合酶活性的鉴定与特性分析
Identification and characterization of an RNA-dependent RNA polymerase activity within the nonstructural protein 5B region of bovine viral diarrhea virus.
作者信息
Zhong W, Gutshall L L, Del Vecchio A M
机构信息
Department of Molecular Virology and Host Defense, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania 19426, USA.
出版信息
J Virol. 1998 Nov;72(11):9365-9. doi: 10.1128/JVI.72.11.9365-9369.1998.
Abstract
Nonstructural protein 5B (NS5B) of bovine viral diarrhea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. We describe the expression and purification of the BVDV NS5B protein derived from an infectious cDNA clone of BVDV (NADL strain). BVDV NS5B protein was active in an in vitro RNA polymerase assay using homopolymeric RNA or BVDV minigenomic RNA templates. The major product was a covalently linked double-stranded molecule generated by a "copy-back" mechanism from the input template RNA. In addition, a nucleotide-nonspecific and template-independent terminal nucleotidyl transferase activity was observed with the BVDV NS5B preparation.
摘要
牛病毒性腹泻病毒（BVDV）的非结构蛋白5B（NS5B）含有可预测RNA依赖性RNA聚合酶活性的序列基序。我们描述了源自BVDV（NADL株）感染性cDNA克隆的BVDV NS5B蛋白的表达和纯化。BVDV NS5B蛋白在使用同聚RNA或BVDV微型基因组RNA模板的体外RNA聚合酶测定中具有活性。主要产物是由输入模板RNA通过“回环复制”机制产生的共价连接的双链分子。此外，在BVDV NS5B制剂中观察到了核苷酸非特异性和模板非依赖性末端核苷酸转移酶活性。
相似文献
1
Identification and characterization of an RNA-dependent RNA polymerase activity within the nonstructural protein 5B region of bovine viral diarrhea virus.牛病毒性腹泻病毒非结构蛋白5B区域内RNA依赖性RNA聚合酶活性的鉴定与特性分析
J Virol. 1998 Nov;72(11):9365-9. doi: 10.1128/JVI.72.11.9365-9369.1998.
2
Mutational analysis of bovine viral diarrhea virus RNA-dependent RNA polymerase.牛病毒性腹泻病毒RNA依赖性RNA聚合酶的突变分析
J Virol. 1999 Dec;73(12):10129-36. doi: 10.1128/JVI.73.12.10129-10136.1999.
3
Generation and characterization of a hepatitis C virus NS3 protease-dependent bovine viral diarrhea virus.一种丙型肝炎病毒NS3蛋白酶依赖性牛病毒性腹泻病毒的产生与特性分析
J Virol. 2000 Jul;74(14):6339-47. doi: 10.1128/jvi.74.14.6339-6347.2000.
4
Infectious bovine viral diarrhea virus (strain NADL) RNA from stable cDNA clones: a cellular insert determines NS3 production and viral cytopathogenicity.来自稳定cDNA克隆的传染性牛病毒性腹泻病毒（NADL株）RNA：一个细胞插入片段决定NS3的产生和病毒细胞致病性。
J Virol. 1998 Jun;72(6):4737-45. doi: 10.1128/JVI.72.6.4737-4745.1998.
5
Involvement of a bovine viral diarrhea virus NS5B locus in virion assembly.牛病毒性腹泻病毒NS5B基因座在病毒粒子组装中的作用。
J Virol. 2004 Sep;78(18):9612-23. doi: 10.1128/JVI.78.18.9612-9623.2004.
6
Specific inhibition of bovine viral diarrhea virus replicase.牛病毒性腹泻病毒复制酶的特异性抑制
J Virol. 2003 Jun;77(12):6753-60. doi: 10.1128/jvi.77.12.6753-6760.2003.
7
The structure of bovine viral diarrhea virus RNA-dependent RNA polymerase and its amino-terminal domain.牛病毒性腹泻病毒RNA依赖的RNA聚合酶的结构及其氨基末端结构域。
Structure. 2006 Jul;14(7):1107-13. doi: 10.1016/j.str.2006.05.020.
8
RNA-dependent RNA polymerase activity encoded by GB virus-B non-structural protein 5B.丙型肝炎病毒B型非结构蛋白5B编码的依赖RNA的RNA聚合酶活性。
J Viral Hepat. 2000 Sep;7(5):335-42. doi: 10.1046/j.1365-2893.2000.00226.x.
9
Ribosomal S27a coding sequences upstream of ubiquitin coding sequences in the genome of a pestivirus.瘟病毒基因组中泛素编码序列上游的核糖体S27a编码序列。
J Virol. 1998 Nov;72(11):8697-704. doi: 10.1128/JVI.72.11.8697-8704.1998.
10
Correlation between point mutations in NS2 and the viability and cytopathogenicity of Bovine viral diarrhea virus strain Oregon analyzed with an infectious cDNA clone.利用感染性 cDNA 克隆分析牛病毒性腹泻病毒俄勒冈株 NS2 点突变与病毒活力及细胞致病性之间的相关性。
J Virol. 2000 Jan;74(1):390-400. doi: 10.1128/jvi.74.1.390-400.2000.
引用本文的文献
1
Proline to Threonine Mutation at Position 162 of NS5B of Classical Swine Fever Virus Vaccine C Strain Promoted Genome Replication and Infectious Virus Production by Facilitating Initiation of RNA Synthesis.经典猪瘟病毒疫苗 C 株 NS5B 蛋白第 162 位脯氨酸到苏氨酸的突变通过促进 RNA 合成起始促进基因组复制和感染性病毒的产生。
Viruses. 2021 Aug 2;13(8):1523. doi: 10.3390/v13081523.
2
Membrane Topology of Pestiviral Non-Structural Protein 2 and determination of the minimal autoprotease domain.瘟病毒非结构蛋白2的膜拓扑结构及最小自蛋白酶结构域的确定
J Virol. 2021 May 10;95(11). doi: 10.1128/JVI.00154-21. Epub 2021 Mar 17.
3
Quinolinecarboxamides Inhibit the Replication of the Bovine Viral Diarrhea Virus by Targeting a Hot Spot for the Inhibition of Pestivirus Replication in the RNA-Dependent RNA Polymerase.喹啉甲酰胺通过靶向 RNA 依赖的 RNA 聚合酶中抑制瘟病毒复制的热点来抑制牛病毒性腹泻病毒的复制。
Molecules. 2020 Mar 12;25(6):1283. doi: 10.3390/molecules25061283.
4
A novel intracellularly expressed NS5B-specific nanobody suppresses bovine viral diarrhea virus replication.一种新型细胞内表达的 NS5B 特异性纳米抗体抑制牛病毒性腹泻病毒复制。
Vet Microbiol. 2020 Jan;240:108449. doi: 10.1016/j.vetmic.2019.108449. Epub 2019 Oct 12.
5
CRISPR/Cas9-Mediated Knockout of DNAJC14 Verifies This Chaperone as a Pivotal Host Factor for RNA Replication of Pestiviruses.CRISPR/Cas9 介导的 DNAJC14 敲除证实该伴侣蛋白是黄病毒 RNA 复制的关键宿主因子。
J Virol. 2019 Feb 19;93(5). doi: 10.1128/JVI.01714-18. Print 2019 Mar 1.
6
Nonreplicative RNA Recombination of an Animal Plus-Strand RNA Virus in the Absence of Efficient Translation of Viral Proteins.动物正链 RNA 病毒在病毒蛋白翻译效率低下的情况下进行非复制性 RNA 重组。
Genome Biol Evol. 2017 Apr 1;9(4):817-829. doi: 10.1093/gbe/evx046.
7
Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.囊状病毒RNA指导的RNA聚合酶：在多个时间和长度尺度上对RNA合成的调控
Virus Res. 2017 Apr 15;234:135-152. doi: 10.1016/j.virusres.2017.01.006. Epub 2017 Jan 16.
8
Purification and Biochemical Characterisation of Rabbit Calicivirus RNA-Dependent RNA Polymerases and Identification of Non-Nucleoside Inhibitors.兔杯状病毒RNA依赖的RNA聚合酶的纯化及生化特性分析与非核苷抑制剂的鉴定
Viruses. 2016 Apr 14;8(4):100. doi: 10.3390/v8040100.
9
Initiation of RNA Polymerization and Polymerase Encapsidation by a Small dsRNA Virus.一种小型双链RNA病毒引发的RNA聚合作用及聚合酶衣壳化
PLoS Pathog. 2016 Apr 14;12(4):e1005523. doi: 10.1371/journal.ppat.1005523. eCollection 2016 Apr.
10
Stability of the resistance to the thiosemicarbazone derived from 5,6-dimethoxy-1-indanone, a non-nucleoside polymerase inhibitor of bovine viral diarrhea virus.源自5,6-二甲氧基-1-茚满酮的硫代氨基脲对牛病毒性腹泻病毒非核苷聚合酶抑制剂的抗性稳定性。
PLoS One. 2014 Jun 20;9(6):e100528. doi: 10.1371/journal.pone.0100528. eCollection 2014.
本文引用的文献
1
Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.丙型肝炎病毒NS5B RNA依赖性RNA聚合酶的生化特性及酶活性必需氨基酸序列基序的鉴定。
J Virol. 1997 Nov;71(11):8416-28. doi: 10.1128/JVI.71.11.8416-8428.1997.
2
Serine protease of pestiviruses: determination of cleavage sites.瘟病毒的丝氨酸蛋白酶：切割位点的确定
J Virol. 1997 Jul;71(7):5415-22. doi: 10.1128/JVI.71.7.5415-5422.1997.
3
Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication.牛病毒性腹泻病毒NS3丝氨酸蛋白酶：多蛋白切割位点、辅因子需求以及瘟病毒复制所必需酶的分子模型
J Virol. 1997 Jul;71(7):5312-22. doi: 10.1128/JVI.71.7.5312-5322.1997.
4
RNA-dependent RNA polymerase of hepatitis C virus.丙型肝炎病毒的RNA依赖性RNA聚合酶
Methods Enzymol. 1996;275:58-67. doi: 10.1016/s0076-6879(96)75006-1.
5
Authentic and chimeric full-length genomic cDNA clones of bovine viral diarrhea virus that yield infectious transcripts.能产生感染性转录本的牛病毒性腹泻病毒的真实和嵌合全长基因组cDNA克隆。
J Virol. 1997 Jan;71(1):471-8. doi: 10.1128/JVI.71.1.471-478.1997.
6
Molecular characterization of pestiviruses.瘟病毒的分子特征
Adv Virus Res. 1996;47:53-118. doi: 10.1016/s0065-3527(08)60734-4.
7
Processing in the pestivirus E2-NS2 region: identification of proteins p7 and E2p7.瘟病毒E2-NS2区域的加工：p7和E2p7蛋白的鉴定
J Virol. 1996 Jun;70(6):4131-5. doi: 10.1128/JVI.70.6.4131-4135.1996.
8
Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus.丙型肝炎病毒RNA依赖性RNA聚合酶的鉴定与特性
EMBO J. 1996 Jan 2;15(1):12-22.
9
Molecular biology of bovine viral diarrhea virus and its interactions with the host.牛病毒性腹泻病毒的分子生物学及其与宿主的相互作用
Vet Clin North Am Food Anim Pract. 1995 Nov;11(3):393-423. doi: 10.1016/s0749-0720(15)30459-x.
10
Processing of the envelope glycoproteins of pestiviruses.瘟病毒包膜糖蛋白的加工过程。
J Virol. 1993 Jun;67(6):3288-94. doi: 10.1128/JVI.67.6.3288-3294.1993.
Zotero 插件