Sentoku N, Tamaoki M, Nishimura A, Matsuoka M
BioScience Center, Nagoya University, Aichi, Japan.
Biochim Biophys Acta. 1998 Aug 20;1399(2-3):203-8. doi: 10.1016/s0167-4781(98)00100-6.
We reported isolation and characterization of a homeobox gene from tobacco, NTH23. The homeodomain structure of NTH23 was highly homologous to the same regions of class 2 genes of the KN1-type homeobox (sharing more than 85% amino acid identity), but was less similar to class 1 genes of KN1-type. RNA gel blot analysis revealed that NTH23 was expressed in all organs we tested although the gene is primarily expressed in young leaves. To determine more precisely the spatial expression pattern of NTH23 in tobacco, a chimeric NTH23::GUS fusion gene was introduced into tobacco. The signal of GUS activity was observed at the basal part of leaf blade primordia in the NTH23::GUS transgenic tobacco plants. This observation suggests the possibility that NTH23 may be important for the lateral growth of leaf blades.
我们报道了从烟草中分离并鉴定出一个同源异型框基因NTH23。NTH23的同源异型结构域与KN1型同源异型框2类基因的相同区域高度同源（氨基酸同一性超过85%），但与KN1型1类基因的相似性较低。RNA凝胶印迹分析表明，尽管该基因主要在幼叶中表达，但在我们测试的所有器官中NTH23均有表达。为了更精确地确定NTH23在烟草中的空间表达模式，将一个嵌合的NTH23::GUS融合基因导入烟草。在NTH23::GUS转基因烟草植株的叶片原基基部观察到了GUS活性信号。这一观察结果提示，NTH23可能对叶片的侧向生长很重要。
