Nishimura A, Tamaoki M, Sakamoto T, Matsuoka M
Nagoya University, BioScience Center, Chikusa, Japan.
Plant Cell Physiol. 2000 May;41(5):583-90. doi: 10.1093/pcp/41.5.583.
We compared the phenotypes of transgenic tobacco plants over-expressing various knotted1-type class1 homeobox genes. All transformants showed abnormal leaf morphology, with the degree of abnormality depending upon the Nicotiana tabacum homeobox (NTH) gene that was over-expressed. Tobacco plants over-expressing NTH1 or NTH9 showed a relatively weak phenotype, while NTH15 and NTH20 over-expressing plants exhibited severe alterations, with occasional ectopic shoot formation on the leaves. Plants over-expressing NTH22 had a relatively severe phenotype, but did not form any ectopic shoots. These results indicate that all of the NTH genes can influence leaf development from the shoot apical meristem, but that the effect varies with the gene. Based on phylogenetic analysis of the NTH genes and comparison of the phenotypes of plants over-expressing them, we suggest that the kn1-type class1 family can be divided into two subgroups, and that the differences in their ability to induce the abnormal phenotype corresponds to the structures of their conserved domains.
我们比较了过量表达各种knotted1类1型同源异型盒基因的转基因烟草植株的表型。所有转化体均表现出异常的叶片形态，异常程度取决于过量表达的烟草同源异型盒（NTH）基因。过量表达NTH1或NTH9的烟草植株表现出相对较弱的表型，而过量表达NTH15和NTH20的植株则表现出严重的变化，叶片上偶尔会形成异位芽。过量表达NTH22的植株具有相对严重的表型，但未形成任何异位芽。这些结果表明，所有NTH基因均可影响茎尖分生组织的叶片发育，但其影响因基因而异。基于NTH基因的系统发育分析以及过量表达这些基因的植株的表型比较，我们认为kn1类1型家族可分为两个亚组，它们诱导异常表型能力的差异与其保守结构域的结构相对应。
