来自龟裂链霉菌R6的recA基因：序列及其在大肠杆菌中的表达

The recA gene from Streptomyces rimosus R6: sequence and expression in Escherichia coli.

作者信息

Mikoc A, Vujaklija D, Gamulin V

机构信息

Department of Molecular Genetics, Ruder Bosković Institute, Zagreb, Croatia.

出版信息

Res Microbiol. 1997 Jun;148(5):397-403. doi: 10.1016/S0923-2508(97)83870-3.

DOI:10.1016/S0923-2508(97)83870-3

PMID:9765818

Abstract

The recA gene from Streptomyces rimusus encodes a 376-amino acids polypeptide (M(r) 39,702) that is one of the largest bacterial RecA proteins observed. Detailed analyses of the Streptomyces RecA proteins showed that all possess an additional and unique C-terminal, rich in lysines and alanines, which can form an additional terminal alpha helix. Expression of the S. rimosus RecA protein in Escherichia coli FR333 (delta recA306) was demonstrated using antibodies raised against E. coli RecA protein; expression was possible only from the S. rimosus promoter. A Streptomyces-E. coli-like promoter sequence (TTGACA-18bp-TCTTAT) was found in the A+ T-rich region 135-165 base pairs upstream from the initiation codon and was related to Bacillus subtilis DNA damage-inducible promoters.

摘要

来自龟裂链霉菌的recA基因编码一种376个氨基酸的多肽（分子量39,702），它是所观察到的最大的细菌RecA蛋白之一。对链霉菌RecA蛋白的详细分析表明，所有这些蛋白都有一个额外的独特的富含赖氨酸和丙氨酸的C末端，它可以形成一个额外的末端α螺旋。使用针对大肠杆菌RecA蛋白产生的抗体，证明了龟裂链霉菌RecA蛋白在大肠杆菌FR333（ΔrecA306）中的表达；只有从龟裂链霉菌启动子才能实现表达。在起始密码子上游135 - 165个碱基对的富含A+T的区域中发现了一个类似链霉菌 - 大肠杆菌的启动子序列（TTGACA - 18bp - TCTTAT），并且它与枯草芽孢杆菌DNA损伤诱导型启动子相关。