Kriukov V M, Zaĭtsev E N, Kuz'min N P, Baev A A
Bioorg Khim. 1990 Sep;16(9):1177-82.
The nucleotide sequence of the 1206 bp fragment of Pseudomonas aeruginosa DNA coding for the recA gene has been determined. This structure was shown to contain an open reading frame corresponding to a protein with m.w. 36808 D highly homologous (70%) to the Escherichia coli recA protein. Homology on the DNA level is significantly lower (57%) due to the high G/C content characteristics of Pseudomonas DNA. Making use of S1 nuclease and reverse transcriptase it was shown that in P. aeruginosa and E. coli cells recAPA gene transcription starts from A or T unit. Unlike "-35" region, "-10" region is homologous to the consensus E. coli promoter sequence. Comparison of primary structures of the recAPA and recAEC proteins demonstrates that the recAPA protein is by 7 amino acid residues shorter and differs from recAEC at 108 positions. Homology is the lowest in the C-terminal part. Basing on the analysis of hybrid recAPA proteins with a modified C-terminal part, it may be suggested that C-terminus is nonessential for main activities of the recA protein.
已确定编码铜绿假单胞菌recA基因的1206 bp片段的核苷酸序列。该结构显示含有一个开放阅读框，对应于一种分子量为36808 D的蛋白质，与大肠杆菌recA蛋白高度同源（70%）。由于铜绿假单胞菌DNA的高G/C含量特征，DNA水平上的同源性显著较低（57%）。利用S1核酸酶和逆转录酶表明，在铜绿假单胞菌和大肠杆菌细胞中，recAPA基因转录从A或T单元开始。与“-35”区域不同，“-10”区域与大肠杆菌启动子共有序列同源。recAPA和recAEC蛋白一级结构的比较表明，recAPA蛋白短7个氨基酸残基，在108个位置与recAEC不同。C末端部分的同源性最低。基于对具有修饰C末端部分的杂合recAPA蛋白的分析，可能表明C末端对于recA蛋白的主要活性是非必需的。
