马赛分枝杆菌新种、蒂蒙分枝杆菌新种和布歇迪尔洪分枝杆菌新种，均为鸟分枝杆菌复合群的成员。
Mycobacterium marseillense sp. nov., Mycobacterium timonense sp. nov. and Mycobacterium bouchedurhonense sp. nov., members of the Mycobacterium avium complex.
作者信息
Ben Salah Iskandar, Cayrou Caroline, Raoult Didier, Drancourt Michel
机构信息
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Université de la Méditerranée, Marseille, France.
出版信息
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2803-8. doi: 10.1099/ijs.0.010637-0. Epub 2009 Jul 23.
An rpoB sequence-based evaluation of 100 Mycobacterium avium complex (MAC) clinical isolates led to the identification of five respiratory tract isolates that were potential representatives of three novel MAC species. Distinctive phenotypic features of isolates 62863 and 5356591(T) included a pseudomycelium morphology and both esterase and acid phosphatase activities. These two isolates exhibited sequence similarities of 99.8 % for the 16S rRNA gene, 86.3 and 86.1 % for 16S-23S rRNA gene internal transcribed spacer (ITS-1) sequence, 96.7 and 97.8 % for rpoB and 97.6 and 97.4 % for hsp65, respectively, with the type strain of Mycobacterium chimaera, the most closely related species. Isolates 3256799 and 5351974(T) lacked alpha-mannosidase and beta-glucosidase activities. They exhibited sequence similarities of 99.6 % for the 16S rRNA gene, 90.1 and 90.4 % for ITS-1, 97.8 % for rpoB and 98.0 and 98.1 % for hsp65, respectively, with the type strain of M. chimaera, the most closely related species. Isolate 4355387(T) lacked urease and alpha-glucosidase activities, but it exhibited valine arylamidase, cystine arylamidase and acid phosphatase activities. It had sequence similarities of 99.3 % for the 16S rRNA gene, 51.8 % for ITS-1, 97.1 % for rpoB and 97.8 % for hsp65 with the type strain of Mycobacterium colombiense, the most closely related species. A phylogenetic tree based on concatenated 16S rRNA gene, ITS-1, rpoB and hsp65 sequences showed the uniqueness of these five isolates as representatives of three novel species, with bootstrap values >/=95 % in all nodes. On the basis of these phenotypic and genetic characteristics, these five isolates are proposed as representatives of three novel MAC species: Mycobacterium marseillense sp. nov., with strain 5356591(T) (=CCUG 56325(T) =CIP 109828(T) =CSUR P30(T)) as the type strain; Mycobacterium timonense sp. nov., with strain 5351974(T) (=CCUG 56329(T) =CIP 109830(T) =CSUR P32(T)) as the type strain; and Mycobacterium bouchedurhonense sp. nov., with strain 4355387(T) (=CCUG 56331(T) =CIP 109827(T) =CSUR P34(T)) as the type strain.
基于rpoB序列对100株鸟分枝杆菌复合群（MAC）临床分离株进行评估，结果鉴定出5株呼吸道分离株，它们可能是3个新的MAC菌种的代表。分离株62863和5356591(T)的独特表型特征包括假菌丝形态以及酯酶和酸性磷酸酶活性。这两株分离株与最密切相关的物种——嵌合体分枝杆菌的模式菌株相比，16S rRNA基因的序列相似性为99.8%，16S - 23S rRNA基因内部转录间隔区（ITS - 1）序列的相似性分别为86.3%和86.1%，rpoB的相似性分别为96.7%和97.8%，hsp65的相似性分别为97.6%和97.4%。分离株3256799和5351974(T)缺乏α - 甘露糖苷酶和β - 葡萄糖苷酶活性。它们与嵌合体分枝杆菌的模式菌株相比，16S rRNA基因的序列相似性为99.6%，ITS - 1的相似性分别为90.1%和90.4%，rpoB的相似性为97.8%，hsp65的相似性分别为98.0%和98.1%。分离株4355387(T)缺乏脲酶和α - 葡萄糖苷酶活性，但具有缬氨酸芳基酰胺酶、胱氨酸芳基酰胺酶和酸性磷酸酶活性。它与最密切相关的物种——哥伦比亚分枝杆菌的模式菌株相比，16S rRNA基因的序列相似性为99.3%，ITS - 1的相似性为51.8%，rpoB的相似性为97.1%，hsp65的相似性为97.8%。基于串联的16S rRNA基因、ITS - 1、rpoB和hsp65序列构建的系统发育树显示，这5株分离株作为3个新物种的代表具有独特性，所有节点的自展值≥95%。基于这些表型和遗传特征，这5株分离株被提议作为3个新的MAC菌种的代表：马赛分枝杆菌，模式菌株为5356591(T)（=CCUG 56325(T)=CIP 109828(T)=CSUR P30(T)）；蒂蒙分枝杆菌，模式菌株为5351974(T)（=CCUG 56329(T)=CIP 109830(T)=CSUR P32(T)）；布歇迪尔洪分枝杆菌，模式菌株为4355387(T)（=CCUG 56331(T)=CIP 109827(T)=CSUR P34(T)）。
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