使用快速检测试剂盒从未经巴氏杀菌的苹果汁中鉴定出单核细胞增生李斯特菌。

Identification of Listeria monocytogenes from unpasteurized apple juice using rapid test kits.

作者信息

Sado P N, Jinneman K C, Husby G J, Sorg S M, Omiecinski C J

机构信息

U. S. Food and Drug Administration, Bothell, Washington 98041, USA.

出版信息

J Food Prot. 1998 Sep;61(9):1199-202. doi: 10.4315/0362-028x-61.9.1199.

DOI:10.4315/0362-028x-61.9.1199

PMID:9766077

Abstract

A microbiological survey of 50 retail juices was conducted in the fall of 1996. These juices were analyzed for Listeria monocytogenes, Escherichia coli O157:H7, Salmonella, coliforms, fecal coliforms, and pH. Two unpasteurized juices were positive for L. monocytogenes: an apple juice and an apple raspberry blend with a pH of 3.78 and 3.75, respectively. Three L. monocytogenes isolates were characterized. The colonies were typical for Listeria sp. on Oxford and lithium chloride-phenylethanol-moxalactam agars and were beta-hemolytic on sheep blood agar. The isolates required 5 days of incubation at 35 degrees C to produce a positive rhamnose reaction in a phenol red carbohydrate broth. This slow rhamnose utilization resulted in these isolates not being identified using the Micro-ID test strip (Organon Technika). However, the isolates were positive for L. monocytogenes using the API Listeria strip (BioMerieux) and a multiplex polymerase chain reaction for detection of the hemolysis (hyla) and invasion-associated protein (iap) genes.

摘要

1996年秋季对50种零售果汁进行了微生物学调查。对这些果汁进行了单核细胞增生李斯特菌、大肠杆菌O157:H7、沙门氏菌、大肠菌群、粪大肠菌群和pH值分析。两种未经巴氏杀菌的果汁单核细胞增生李斯特菌呈阳性：一种苹果汁和一种苹果树莓混合汁，pH值分别为3.78和3.75。对三株单核细胞增生李斯特菌分离株进行了鉴定。这些菌落在牛津琼脂和氯化锂-苯乙醇-莫拉酰胺琼脂上是李斯特菌属的典型菌落，在绵羊血琼脂上呈β溶血。这些分离株在35℃下培养5天才能在酚红碳水化合物肉汤中产生阳性鼠李糖反应。这种缓慢的鼠李糖利用导致这些分离株无法使用Micro-ID试纸条（奥加农技术公司）进行鉴定。然而，使用API李斯特菌试纸条（生物梅里埃公司）以及用于检测溶血（hyla）和侵袭相关蛋白（iap）基因的多重聚合酶链反应，这些分离株对单核细胞增生李斯特菌呈阳性。