单核细胞增生李斯特菌IIIA、IIIB和IIIC亚群描绘了具有不同致病潜力的基因上不同的群体。
Listeria monocytogenes subgroups IIIA, IIIB, and IIIC delineate genetically distinct populations with varied pathogenic potential.
作者信息
Liu Dongyou, Lawrence Mark L, Wiedmann Martin, Gorski Lisa, Mandrell Robert E, Ainsworth A Jerald, Austin Frank W
机构信息
College of Veterinary Medicine, Mississippi State University, P.O. Box 6100, Mississippi State, MS 39762, USA.
出版信息
J Clin Microbiol. 2006 Nov;44(11):4229-33. doi: 10.1128/JCM.01032-06. Epub 2006 Sep 27.
Abstract
Listeria monocytogenes lineage III strains belonging to subgroups IIIA (n = 8), IIIB (n = 5), and IIIC (n = 6) were examined along with other known serotype strains (n = 11) by PCR and Southern hybridization using several recently described species-, virulence-, and serotype-specific primers and probes. The virulence of seven representative lineage III strains was then evaluated in mice via the intraperitoneal route. The results suggest that subgroup IIIA consists of typical rhamnose-positive avirulent serotype 4a and virulent serotype 4c strains, subgroup IIIC consists of atypical rhamnose-negative virulent serotype 4c strains, and subgroup IIIB consists of atypical rhamnose-negative virulent non-serotype 4a and non-serotype 4c strains, some of which may be related to serotype 7. It is possible that subgroup IIIB (including serotype 7) may represent a novel subspecies within L. monocytogenes.
摘要
使用几种最近描述的物种特异性、毒力特异性和血清型特异性引物及探针，通过聚合酶链反应（PCR）和Southern杂交，对属于IIIA亚组（n = 8）、IIIB亚组（n = 5）和IIIC亚组（n = 6）的单核细胞增生李斯特菌III型菌株以及其他已知血清型菌株（n = 11）进行了检测。然后，通过腹腔注射途径在小鼠中评估了7株代表性III型菌株的毒力。结果表明，IIIA亚组由典型的鼠李糖阳性无毒血清型4a和有毒血清型4c菌株组成，IIIC亚组由非典型的鼠李糖阴性有毒血清型4c菌株组成，IIIB亚组由非典型的鼠李糖阴性有毒非血清型4a和非血清型4c菌株组成，其中一些可能与血清型7有关。IIIB亚组（包括血清型7）有可能代表单核细胞增生李斯特菌内的一个新亚种。
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