McNerney R, Wilson S M, Sidhu A M, Harley V S, al Suwaidi Z, Nye P M, Parish T, Stoker N G
Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine.
Res Microbiol. 1998 Jul-Aug;149(7):487-95. doi: 10.1016/s0923-2508(98)80003-x.
There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have successfully infected cells, a key step is the efficient removal or inactivation of phages remaining free in the culture medium. We demonstrate here the use of ferrous ammonium sulphate as an effective agent for the inactivation of mycobacteriophage D29 without impairing phage replication in previously infected host bacteria. Using this property, we report the detection of viable Mycobacterium smegmatis, M. bovis BCG and M. tuberculosis using simple low-cost technology. The method is highly sensitive, since it is able to detect 10 colony-forming units of M. smegmatis. It is also rapid, with the detection of M. tuberculosis in sputum specimens within 48 h.
对于临床样本中分枝杆菌的检测和药敏试验，仍然迫切需要更灵敏、更具成本效益的方法。我们一直在研究一种基于噬菌体的简单系统，该系统可用于这两个目的。由于这依赖于对成功感染细胞的噬菌体的检测，关键步骤是有效去除或灭活培养基中残留的游离噬菌体。我们在此证明硫酸亚铁铵作为一种有效试剂可灭活分枝杆菌噬菌体D29，而不影响噬菌体在先前感染的宿主细菌中的复制。利用这一特性，我们报告了使用简单低成本技术检测活的耻垢分枝杆菌、卡介苗和结核分枝杆菌。该方法高度灵敏，因为它能够检测到10个耻垢分枝杆菌菌落形成单位。它也很快速，能在48小时内检测痰标本中的结核分枝杆菌。
