不同专业化程度的鸽类红细胞中重复和独特DNA核苷酸序列的转录

Transcription of repetitive and unique DNA nucleotide sequences in pigeon erythroid cells with different degrees of specialization.

作者信息

Gasaryan K G, Tarantul W Z, Baranov Y N

出版信息

Differentiation. 1976 Mar 16;6(1):41-6. doi: 10.1111/j.1432-0436.1976.tb01466.x.

DOI:10.1111/j.1432-0436.1976.tb01466.x

Abstract

HnRNA fractions with sedimentation coefficients greater than 45 S isolated from pigeon bone marrow as well as from the immature and mature erythroid cells of periferal blood were hybridised with a large excess of DNA fractionated on the basis of renaturation kinetics. 58-62% of the input RNAs were recovered as RNAase-resistant hybrids. About 1/3 (20%) of bone marrow greater than 45 S RNA found in the hybrids was hybridised with the repetitive and baout 2/3 (40%) with the unique DNA sequences. In addition, a considerably smaller portion of greater than 45 S RNA from the "reticulocytes" (13%) and "erythrocytes" (approximately 6%) was hybridised with the repetitive DNA.

摘要

从鸽骨髓以及外周血未成熟和成熟红细胞中分离出沉降系数大于45S的核不均一RNA（hnRNA）组分，使其与根据复性动力学分级分离的大量过量DNA杂交。58 - 62%的输入RNA以抗核糖核酸酶杂交体的形式回收。杂交体中发现的大于45S的骨髓RNA约1/3（20%）与重复DNA杂交，约2/3（40%）与单一DNA序列杂交。此外，来自“网织红细胞”（13%）和“红细胞”（约6%）的大于45S RNA与重复DNA杂交的部分要小得多。