鸽基因组中两个转录重复DNA片段的纯化与特性分析

Purification and characterization of two transcribed repetitive DNA fractions from the pigeon genome.

作者信息

Gasaryan K G, Kuznetsova E D, Tarantul V Z, Sivak S A

出版信息

Chromosoma. 1977 Jun 23;61(4):381-94. doi: 10.1007/BF00288621.

Abstract

Two fractions of the repeats belonging to intermediate frequency repetitive DNA were isolated from the total pigeon nuclear DNA fragmented to about 450 nucleotides. One fraction was designated as "rare repeats" (repetition frequency about 35 per haploid genome) and another termed as "moderate repeats" (repetition frequency about 2500 per haploid genome). The rare repeats, which constitute about 7% of the total DNA, include at least 75% of the repetitive DNA sequences transcribed into the high molecular fraction (greater than 45S) of HnRNA in erythroid cells. These repeats have properties compatible with the characteristics of the class of low frequency interspersed DNA found in genomes of many other Metazoan species. The moderate repeats contribute only about 10-20% of the total repetitive DNA copies present in greater than 45S HnRNA and differ from the rare repeats in some other properties.--The possible role of the rare repeats in the genome is discussed.

摘要

从片段化至约450个核苷酸的鸽总核DNA中分离出属于中频重复DNA的两个重复序列组分。一个组分被指定为“稀有重复序列”（单倍体基因组中的重复频率约为35次），另一个被称为“中度重复序列”（单倍体基因组中的重复频率约为2500次）。稀有重复序列约占总DNA的7%，其中至少75%的重复DNA序列转录到红细胞中高分子量部分（大于45S）的核不均一RNA中。这些重复序列具有与许多其他后生动物物种基因组中发现的低频散布DNA类别的特征相符的特性。中度重复序列仅占大于45S核不均一RNA中存在的总重复DNA拷贝的约10 - 20%，并且在其他一些特性上与稀有重复序列不同。讨论了稀有重复序列在基因组中的可能作用。