Carrick C S, Fyfe J A, Davies J K
Department of Microbiology, Monash University, Clayton, Victoria, 3168, Australia.
Gene. 1998 Oct 5;220(1-2):21-9. doi: 10.1016/s0378-1119(98)00424-7.
A 960-bp ORF potentially encoding a site-specific recombinase has been cloned from Neisseria gonorrhoeae MS11-A. This ORF was designated pivNg on the basis of similarity of the deduced amino acid sequence to the Piv proteins of Moraxella spp. that are site-specific invertases. Southern hybridization and sequence analysis revealed that there were multiple copies of pivNg sequence within the genomes of N. gonorrhoeae strains tested, but not in several other neisserial species. Southern hybridization and sequence analysis further suggested that pivNg sequences may be associated with genomic rearrangements.
从淋病奈瑟菌MS11-A中克隆出一个960bp的开放阅读框（ORF），它可能编码一种位点特异性重组酶。基于推导的氨基酸序列与莫拉克斯氏菌属的Piv蛋白（即位点特异性转化酶）的相似性，该ORF被命名为pivNg。Southern杂交和序列分析表明，在所测试的淋病奈瑟菌菌株基因组中存在多个pivNg序列拷贝，但在其他几种奈瑟菌属物种中则没有。Southern杂交和序列分析进一步表明，pivNg序列可能与基因组重排有关。
