Rozsa F W, Meyer T F, Fussenegger M
Department of Microbiology, Biozentrum, University of Basel, Switzerland.
J Bacteriol. 1997 Apr;179(7):2382-8. doi: 10.1128/jb.179.7.2382-2388.1997.
A plasmid library of Neisseria gonorrhoeae sequences was screened for the ability to mediate recombinations on a sequence containing the Moraxella lacunata type 4 pilin gene invertible region in Escherichia coli. A plasmid containing the N. gonorrhoeae sequence encoding the putative recombinase (gcr) was identified and sequenced. Plasmids containing gcr were able to mediate site-specific recombinations despite a weak amino acid homology to Piv, the native M. lacunata pilin gene invertase. The gcr gene is present only in pathogenic strains of Neisseria tested; however, in our assays gene knockouts of gcr did not alter the variation of surface features that play a role in the pathogenesis of N. gonorrhoeae.
对淋病奈瑟菌序列的质粒文库进行筛选，以检测其在含有迟钝莫拉菌4型菌毛蛋白基因可变区的序列上介导大肠杆菌重组的能力。鉴定并测序了一个含有编码假定重组酶（gcr）的淋病奈瑟菌序列的质粒。尽管与天然迟钝莫拉菌菌毛蛋白基因转化酶Piv的氨基酸同源性较弱，但含有gcr的质粒仍能够介导位点特异性重组。gcr基因仅存在于所检测的淋病奈瑟菌致病菌株中；然而，在我们的试验中，gcr基因敲除并未改变在淋病奈瑟菌发病机制中起作用的表面特征的变异。
