Lei B, Adachi N, Arai T
Department of Anesthesiology and Resuscitology, Ehime University School of Medicine, Shitsukawa, Shigenobu-cho, Onsen-gun, Ehime 791-0295, Japan.
Brain Res Brain Res Protoc. 1998 Sep;3(1):33-6. doi: 10.1016/s1385-299x(98)00018-x.
This paper reports on the protocol for the determination of H2O2 in the brain using in vivo microdialysis coupled with fluorometry of dichlorofluorescin oxidation. We applied this protocol to monitor changes in the concentration of H2O2 in the brain, in vivo, during ischemia and reperfusion. Using this method, changes in the level of H2O2 in the brain during ischemia and reperfusion were effectively determined. The present protocol provides a novel tool to study the production of reactive oxygen species in the brain.
本文报道了一种使用体内微透析结合二氯荧光素氧化荧光测定法测定脑中过氧化氢（H2O2）的实验方案。我们应用该方案在体内监测缺血和再灌注过程中脑中H2O2浓度的变化。使用这种方法，有效测定了缺血和再灌注过程中脑中H2O2水平的变化。本实验方案为研究脑中活性氧的产生提供了一种新工具。
