State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian, 116024, People's Republic of China.
J Fluoresc. 2013 Nov;23(6):1113-20. doi: 10.1007/s10895-013-1240-8. Epub 2013 Jun 2.
Nitric oxide (NO) is one of the most important intercellular signaling molecules, and plays important roles in various biological systems. In this work, a unique Ru(II) complex, tris[(5-(4-methylamino-3-aminobenzylamino)-1,10-phenanthroline)] ruthenium(II) hexafluorophosphate [Ru(MAA-phen)3][PF6]2, has been designed and synthesized as a luminescent probe for the detection of NO in aqueous media. The complex itself is almost non-luminescent, but can specifically react with NO under the aerobic conditions to afford its highly luminescent triazole derivative in aqueous media, Ru(MTA-phen)3 (MTA-phen: methyl-trazolebenzylamino-1,10-phenanthroline), accompanied by a 302-fold increase in luminescence intensity at 598 nm with a 130 nm Stokes shift. The luminescence response of Ru(MAA-phen)3 to NO is rapid, highly specific without interferences of other reactive oxygen/nitrogen species, and highly stable against the pH changes in the range of pH 4.5-9.5. These features enable Ru(MAA-phen)3 to be used as a probe for the highly selective and sensitive luminescence detection of NO in weakly acidic, neutral, and weakly basic media.
一氧化氮（NO）是最重要的细胞间信号分子之一，在各种生物系统中发挥着重要作用。在这项工作中，设计并合成了一种独特的钌（II）配合物，三[（5-（4-甲氨基-3-氨甲基氨基）-1,10-菲啰啉）]六氟合钌（II）[PF6]2 [Ru（MAA-phen）3][PF6]2，作为在水介质中检测 NO 的荧光探针。该配合物本身几乎没有发光，但可以在有氧条件下特异性地与 NO 反应，在水介质中生成其高发光的三唑衍生物[Ru（MTA-phen）3]（2+）（MTA-phen：甲基-三唑-苯甲氨基-1,10-菲啰啉），同时在 598nm 处的荧光强度增加了 302 倍，Stokes 位移为 130nm。[Ru（MAA-phen）3]（2+）对 NO 的荧光响应快速、高度特异性，不受其他活性氧/氮物质的干扰，并且在 pH 4.5-9.5 的范围内对 pH 变化高度稳定。这些特性使 [Ru（MAA-phen）3]（2+）能够在弱酸性、中性和弱碱性介质中作为探针，用于高度选择性和灵敏的 NO 荧光检测。
